Experiment set7IT023 for Pseudomonas fluorescens FW300-N2E2

LB with Fusidic acid sodium salt 0.4 mg/ml

Group: stress
Media: LB + Fusidic acid sodium salt (0.4 mg/ml)
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Mark on 2/1/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 15 genes in this experiment

For stress Fusidic acid sodium salt in Pseudomonas fluorescens FW300-N2E2

For stress Fusidic acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	1
ATP-dependent RNA helicases, bacterial	1
Carboxysome	1
Copper homeostasis	1
DNA-replication	1
DNA repair, bacterial RecFOR pathway	1
Glycine and Serine Utilization	1
Heat shock dnaK gene cluster extended	1
Lipid A modifications	1
Methionine Biosynthesis	1
Peptidoglycan Biosynthesis	1
Periplasmic disulfide interchange	1
Pyridoxin (Vitamin B6) Biosynthesis	1
Respiratory dehydrogenases 1	1
Serine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• Benzoxazinone biosynthesis
• Ubiquinone and menaquinone biosynthesis
• Oxidative phosphorylation
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Peptidoglycan biosynthesis
• Naphthalene and anthracene degradation
• Porphyrin and chlorophyll metabolism
• Diterpenoid biosynthesis
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
NADH to cytochrome bo oxidase electron transfer I	2	2	1
NADH to cytochrome bd oxidase electron transfer I	2	1	1
aerobic respiration III (alternative oxidase pathway)	3	3	1
L-serine biosynthesis I	3	3	1
L-cysteine biosynthesis IX (Trichomonas vaginalis)	3	2	1
superpathway of L-serine and glycine biosynthesis I	4	4	1
aerobic respiration I (cytochrome c)	4	3	1
NAD(P)/NADPH interconversion	6	3	1
Fe(II) oxidation	6	3	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	2
superpathway of sulfate assimilation and cysteine biosynthesis	9	9	1
peptidoglycan biosynthesis II (staphylococci)	17	12	1
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	1