Experiment set7IT023 for Pseudomonas syringae pv. syringae B728a
a-Ketoglutaric acid disodium salt hydrate carbon source
Group: carbon sourceMedia: M9 minimal media_noCarbon + a-Ketoglutaric acid disodium salt hydrate (21.04598548 mM)
Culturing: SyringaeB728a_ML2, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler Helmann on 1/6/19
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride
Specific Phenotypes
For 11 genes in this experiment
For carbon source a-Ketoglutaric acid disodium salt hydrate across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Arginine and proline metabolism
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
- Fatty acid biosynthesis
- Pyrimidine metabolism
- Lysine degradation
- Histidine metabolism
- Tyrosine metabolism
- Phenylalanine metabolism
- Nucleotide sugars metabolism
- Lipopolysaccharide biosynthesis
- Glycerophospholipid metabolism
- Ether lipid metabolism
- Glycosphingolipid biosynthesis - ganglio series
- Pyruvate metabolism
- 1- and 2-Methylnaphthalene degradation
- Benzoate degradation via CoA ligation
- Ethylbenzene degradation
- Butanoate metabolism
- Carbon fixation in photosynthetic organisms
- Limonene and pinene degradation
- Diterpenoid biosynthesis
- Carotenoid biosynthesis - General
- Anthocyanin biosynthesis
- Alkaloid biosynthesis I
- Alkaloid biosynthesis II
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of type II polyketide backbone
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from terpenoid and polyketide
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-mannose degradation II | 2 | 1 | 1 |
| pseudouridine degradation | 2 | 1 | 1 |
| D-mannose degradation I | 2 | 1 | 1 |
| L-arginine degradation II (AST pathway) | 5 | 5 | 2 |
| β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation | 3 | 2 | 1 |
| mannitol biosynthesis | 3 | 1 | 1 |
| C4 photosynthetic carbon assimilation cycle, NADP-ME type | 7 | 4 | 2 |
| GDP-mannose biosynthesis | 4 | 4 | 1 |
| mannitol degradation II | 4 | 2 | 1 |
| 1,5-anhydrofructose degradation | 5 | 2 | 1 |
| methylgallate degradation | 6 | 2 | 1 |
| C4 photosynthetic carbon assimilation cycle, PEPCK type | 14 | 9 | 2 |
| β-(1,4)-mannan degradation | 7 | 2 | 1 |
| protocatechuate degradation I (meta-cleavage pathway) | 8 | 3 | 1 |
| superpathway of vanillin and vanillate degradation | 10 | 4 | 1 |
| colanic acid building blocks biosynthesis | 11 | 9 | 1 |
| syringate degradation | 12 | 3 | 1 |
| gluconeogenesis I | 13 | 13 | 1 |
| superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis | 14 | 7 | 1 |