Experiment set7IT021 for Pseudomonas fluorescens FW300-N2E2

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LB with Sodium Chloride 600 mM

Group: stress
Media: LB + Sodium Chloride (600 mM)
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Mark on 2/1/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 14 genes in this experiment

For stress Sodium Chloride in Pseudomonas fluorescens FW300-N2E2

For stress Sodium Chloride across organisms

SEED Subsystems

Subsystem #Specific
Choline and Betaine Uptake and Betaine Biosynthesis 6
Bacterial Chemotaxis 1
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. 1
DNA Repair Base Excision 1
DNA repair, UvrABC system 1
DNA repair, bacterial DinG and relatives 1
Flagellar motility 1
Folate Biosynthesis 1
Lipid A modifications 1
Peptidoglycan Biosynthesis 1
Tryptophan synthesis 1
Two-component regulatory systems in Campylobacter 1
Type IV pilus 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamine degradation I 1 1 1
choline degradation I 2 2 1
L-glutamate biosynthesis I 2 2 1
glycine betaine biosynthesis I (Gram-negative bacteria) 2 2 1
glycine betaine biosynthesis II (Gram-positive bacteria) 2 2 1
4-aminobenzoate biosynthesis I 2 2 1
β-alanine biosynthesis I 2 1 1
β-alanine biosynthesis IV 2 1 1
ammonia assimilation cycle III 3 3 1
choline-O-sulfate degradation 3 3 1
glycine betaine biosynthesis III (plants) 3 2 1
dimethylsulfoniopropanoate biosynthesis I (Wollastonia) 3 1 1
L-asparagine biosynthesis III (tRNA-dependent) 4 4 1
glutaminyl-tRNAgln biosynthesis via transamidation 4 4 1
choline degradation IV 4 2 1
dimethylsulfoniopropanoate biosynthesis II (Spartina) 4 1 1
L-glutamate and L-glutamine biosynthesis 7 6 1
L-citrulline biosynthesis 8 6 1
peptidoglycan biosynthesis IV (Enterococcus faecium) 17 12 2
peptidoglycan biosynthesis II (staphylococci) 17 12 2
peptidoglycan biosynthesis V (β-lactam resistance) 17 11 2
chloramphenicol biosynthesis 9 1 1
superpathway of tetrahydrofolate biosynthesis 10 8 1
superpathway of candicidin biosynthesis 11 4 1
peptidoglycan biosynthesis I (meso-diaminopimelate containing) 12 12 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 10 1
superpathway of L-citrulline metabolism 12 8 1
peptidoglycan maturation (meso-diaminopimelate containing) 12 4 1
peptidoglycan biosynthesis III (mycobacteria) 15 11 1
superpathway of chorismate metabolism 59 43 1