Experiment set7IT021 for Bacteroides thetaiotaomicron VPI-5482

Varel_Bryant medium with Chlorpromazine hydrochloride 0.05 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Chlorpromazine hydrochloride (0.05 mM) + Dimethyl Sulfoxide (0.5 vol%)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Adam on 5/2/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: 2029 C2

Specific Phenotypes

For 5 genes in this experiment

For stress Chlorpromazine hydrochloride in Bacteroides thetaiotaomicron VPI-5482

For stress Chlorpromazine hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
Fatty Acid Biosynthesis FASII	1
Pyruvate metabolism I: anaplerotic reactions, PEP	1
mycolic acid synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid biosynthesis
• Pyruvate metabolism
• Carbon fixation in photosynthetic organisms
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from terpenoid and polyketide

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
bile acids 7-O epimerization	4	2	2
ursodeoxycholate biosynthesis (bacteria)	2	1	1
C4 photosynthetic carbon assimilation cycle, NADP-ME type	7	4	2
gondoate biosynthesis (anaerobic)	4	4	1
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast)	12	9	3
palmitate biosynthesis III	29	21	7
tetradecanoate biosynthesis (mitochondria)	25	17	6
palmitate biosynthesis II (type II fatty acid synthase)	31	29	7
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate)	9	8	2
oleate biosynthesis IV (anaerobic)	14	13	3
superpathway of fatty acids biosynthesis (E. coli)	53	47	11
fatty acid elongation -- saturated	5	5	1
superpathway of unsaturated fatty acids biosynthesis (E. coli)	20	17	4
cis-vaccenate biosynthesis	5	4	1
8-amino-7-oxononanoate biosynthesis IV	5	4	1
superpathway of fatty acid biosynthesis II (plant)	43	37	8
8-amino-7-oxononanoate biosynthesis I	11	9	2
odd iso-branched-chain fatty acid biosynthesis	34	24	6
anteiso-branched-chain fatty acid biosynthesis	34	24	6
even iso-branched-chain fatty acid biosynthesis	34	24	6
stearate biosynthesis II (bacteria and plants)	6	5	1
(5Z)-dodecenoate biosynthesis I	6	5	1
stearate biosynthesis IV	6	4	1
(5Z)-dodecenoate biosynthesis II	6	4	1
methylgallate degradation	6	2	1
petroselinate biosynthesis	6	2	1
bile acids epimerization	12	2	2
streptorubin B biosynthesis	34	20	5
C4 photosynthetic carbon assimilation cycle, PEPCK type	14	7	2
biotin biosynthesis I	15	13	2
superpathway of fatty acid biosynthesis I (E. coli)	16	14	2
protocatechuate degradation I (meta-cleavage pathway)	8	3	1
2-allylmalonyl-CoA biosynthesis	8	2	1
superpathway of vanillin and vanillate degradation	10	3	1
syringate degradation	12	3	1
gluconeogenesis I	13	11	1
mycolate biosynthesis	205	18	5
superpathway of mycolate biosynthesis	239	19	5