Experiment set7IT018 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Nickel (II) chloride hexahydrate 0.5 mM

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_5036 +1.3 9.4 Cobalt-zinc-cadmium resistance protein conserved
Pf6N2E2_1396 +1.3 2.1 ABC transporter, ATP-binding protein compare
Pf6N2E2_241 +1.2 2.1 hypothetical protein compare
Pf6N2E2_1147 +1.1 2.9 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) compare
Pf6N2E2_5666 +1.1 6.1 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_5665 +1.0 6.1 Succinylornithine transaminase (EC 2.6.1.81) compare
Pf6N2E2_180 +1.0 4.4 Transcriptional regulator, MerR family compare
Pf6N2E2_961 +1.0 3.2 FIG00954712: hypothetical protein compare
Pf6N2E2_2413 +0.9 1.8 FIG074102: hypothetical protein compare
Pf6N2E2_4803 +0.9 1.6 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_5972 +0.8 2.7 hypothetical protein compare
Pf6N2E2_2984 +0.8 1.7 LysR family transcriptional regulator PA3398 compare
Pf6N2E2_3665 +0.8 1.1 serine/threonine protein kinase compare
Pf6N2E2_5086 +0.8 1.3 hypothetical protein compare
Pf6N2E2_3641 +0.8 2.8 ADP-ribose pyrophosphatase (EC 3.6.1.13) compare
Pf6N2E2_4615 +0.8 1.2 type IV pili signal transduction protein PilI compare
Pf6N2E2_3835 +0.8 3.6 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf6N2E2_3634 +0.7 2.0 Transporter compare
Pf6N2E2_3328 +0.7 1.7 transcriptional regulator compare
Pf6N2E2_2459 +0.7 2.4 Organic hydroperoxide resistance protein compare
Pf6N2E2_5323 +0.7 2.9 FIG00959654: hypothetical protein compare
Pf6N2E2_919 +0.7 0.9 FIG00954373: hypothetical protein compare
Pf6N2E2_1508 +0.7 2.1 DNA-binding response regulator compare
Pf6N2E2_4570 +0.7 1.6 Cation/multidrug efflux pump compare
Pf6N2E2_1683 +0.7 1.8 Fe2+-dicitrate sensor, membrane component compare
Pf6N2E2_451 +0.7 2.1 transcriptional regulator MvaT, P16 subunit, putative compare
Pf6N2E2_5794 +0.7 1.8 Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_1031 +0.7 2.5 Alkanesulfonates transport system permease protein compare
Pf6N2E2_1984 +0.7 3.0 probable exported protein STY4558 compare
Pf6N2E2_1860 +0.7 2.7 Ferredoxin, 2Fe-2S compare
Pf6N2E2_4362 +0.7 2.0 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_5375 +0.7 2.5 Oxidoreductase, short chain dehydrogenase/reductase family compare
Pf6N2E2_2281 +0.7 1.1 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_1288 +0.7 1.4 Protein containing transglutaminase-like domain, putative cysteine protease compare
Pf6N2E2_6087 +0.7 1.3 Short-chain dehydrogenase/reductase SDR compare
Pf6N2E2_1032 +0.7 1.1 Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB compare
Pf6N2E2_1759 +0.7 1.9 uncharacterized domain 1 compare
Pf6N2E2_1315 +0.7 1.0 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) compare
Pf6N2E2_1403 +0.7 2.0 Nitrous oxide reductase maturation protein, outer-membrane lipoprotein NosL compare
Pf6N2E2_737 +0.7 1.5 Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4) compare
Pf6N2E2_5712 +0.7 2.5 DNA-3-methyladenine glycosylase (EC 3.2.2.20) compare
Pf6N2E2_3096 +0.7 1.2 Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_3475 +0.7 1.8 hypothetical protein compare
Pf6N2E2_474 +0.7 2.9 Transcriptional regulator, ArsR family compare
Pf6N2E2_4277 +0.7 5.4 LysR family transcriptional regulator PA5437 compare
Pf6N2E2_3480 +0.7 1.4 Transcriptional regulator, ArsR family compare
Pf6N2E2_4110 +0.7 2.7 FIG01201438: hypothetical protein compare
Pf6N2E2_3346 +0.6 2.9 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf6N2E2_2894 +0.6 3.4 N-acylglucosamine 2-epimerase (EC 5.1.3.8) compare
Pf6N2E2_3825 +0.6 3.2 Nitrogen regulation protein NtrB (EC 2.7.13.3) compare
Pf6N2E2_642 +0.6 2.1 Sigma factor regulator VreR (cytoplasmic membrane-localized) of trans-envelope signaling system compare
Pf6N2E2_2124 +0.6 1.3 Mg(2+) transport ATPase protein C compare
Pf6N2E2_4423 +0.6 2.3 FIG00953342: hypothetical protein compare
Pf6N2E2_4478 +0.6 3.9 FIG019278: hypothetical protein compare
Pf6N2E2_3325 +0.6 1.7 ABC transporter, ATP-binding protein compare
Pf6N2E2_3151 +0.6 1.6 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_1673 +0.6 1.7 Transcriptional regulator, TetR family compare
Pf6N2E2_5133 +0.6 2.0 FIG00953980: hypothetical protein compare
Pf6N2E2_1136 +0.6 1.4 Transcriptional regulator, TetR family compare
Pf6N2E2_4008 +0.6 2.8 hypothetical protein compare
Pf6N2E2_3663 +0.6 4.6 Glycosyl transferase compare
Pf6N2E2_6007 +0.6 1.9 Glutathione S-transferase (EC 2.5.1.18) compare
Pf6N2E2_5001 +0.6 1.3 D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4) compare
Pf6N2E2_4527 +0.6 1.6 RNA polymerase sigma-70 factor, ECF subfamily compare
Pf6N2E2_4068 +0.6 4.4 Phosphate regulon metal ion transporter containing CBS domains compare
Pf6N2E2_5236 +0.6 1.5 hypothetical protein compare
Pf6N2E2_3550 +0.6 2.1 Urea ABC transporter, ATPase protein UrtD compare
Pf6N2E2_2181 +0.6 1.4 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_2788 +0.6 1.4 Transcriptional regulator, AsnC family compare
Pf6N2E2_2123 +0.6 2.1 hypothetical protein compare
Pf6N2E2_2508 +0.6 1.5 hypothetical protein compare
Pf6N2E2_5219 +0.6 3.6 FIG140336: TPR domain protein compare
Pf6N2E2_1092 +0.6 2.0 Probable vanillate O-demethylase oxygenase subunit oxidoreductase protein (EC 1.14.13.-) compare
Pf6N2E2_5037 +0.6 1.8 Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase) compare
Pf6N2E2_226 +0.6 1.6 2,3-dihydroxybiphenyl 1,2-dioxygenase compare
Pf6N2E2_2526 +0.6 3.1 Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase compare
Pf6N2E2_4278 +0.6 4.3 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf6N2E2_2375 +0.6 1.5 Response regulator NasT compare
Pf6N2E2_5910 +0.6 1.4 Flp pilus assembly protein RcpC/CpaB compare
Pf6N2E2_1424 +0.6 1.2 transcriptional regulator, TetR family compare
Pf6N2E2_5906 +0.6 1.7 Flp pilus assembly protein, pilin Flp compare
Pf6N2E2_55 +0.6 1.1 Probable hydrolase compare
Pf6N2E2_98 +0.5 0.9 putative transmembrane protein compare
Pf6N2E2_4710 +0.5 1.4 hypothetical protein compare
Pf6N2E2_1997 +0.5 2.1 FIG00956406: hypothetical protein compare
Pf6N2E2_3285 +0.5 1.7 Rod shape-determining protein MreD compare
Pf6N2E2_5251 +0.5 2.4 FIG00954698: hypothetical protein compare
Pf6N2E2_5576 +0.5 2.0 Cys-tRNA(Pro) deacylase YbaK compare
Pf6N2E2_4467 +0.5 1.6 hypothetical protein compare
Pf6N2E2_1754 +0.5 1.3 FIG00731982: hypothetical protein compare
Pf6N2E2_170 +0.5 1.4 lipoprotein, putative compare
Pf6N2E2_1784 +0.5 0.8 Methyl-accepting chemotaxis protein compare
Pf6N2E2_5730 +0.5 1.4 FIG00954480: hypothetical protein compare
Pf6N2E2_4188 +0.5 1.6 DNA-3-methyladenine glycosylase (EC 3.2.2.20) compare
Pf6N2E2_4543 +0.5 1.7 hypothetical protein compare
Pf6N2E2_1779 +0.5 1.3 Positive regulator of CheA protein activity (CheW) compare
Pf6N2E2_2083 +0.5 1.9 hypothetical protein compare
Pf6N2E2_1161 +0.5 2.0 FIG00961921: hypothetical protein compare
Pf6N2E2_2912 +0.5 1.1 FIG00954094: hypothetical protein compare
Pf6N2E2_4505 +0.5 2.4 Alkanesulfonates transport system permease protein compare
Pf6N2E2_2447 +0.5 4.1 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf6N2E2_101 +0.5 1.5 CigR (Putative inner membrane protein) compare
Pf6N2E2_5816 +0.5 1.4 Smr domain protein compare
Pf6N2E2_2519 +0.5 0.8 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_5061 +0.5 1.8 Quinate/shikimate 5-dehydrogenase I delta (EC 1.1.1.25) compare
Pf6N2E2_2872 +0.5 3.7 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf6N2E2_3656 +0.5 2.7 Probable transcription regulator Mig-14 compare
Pf6N2E2_4917 +0.5 1.5 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_1624 +0.5 1.3 MbtH-like protein compare
Pf6N2E2_3192 +0.5 1.8 tRNA:Cm32/Um32 methyltransferase compare
Pf6N2E2_168 +0.5 2.6 hypothetical protein compare
Pf6N2E2_2594 +0.5 2.7 Flagellar hook-basal body complex protein FliE compare
Pf6N2E2_2869 +0.5 1.0 Peptide deformylase (EC 3.5.1.88) compare
Pf6N2E2_2405 +0.5 2.9 Hypothetical protein PvdY @ Siderophore synthetase small component, acetyltransferase compare
Pf6N2E2_5920 +0.5 1.7 Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly compare
Pf6N2E2_5460 +0.5 2.5 Phage protein compare
Pf6N2E2_5237 +0.5 0.9 Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) compare
Pf6N2E2_5986 +0.5 1.3 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_2798 +0.5 1.5 Death on curing protein, Doc toxin compare
Pf6N2E2_4540 +0.5 1.5 hypothetical protein compare
Pf6N2E2_2681 +0.5 2.1 3-phosphoglycerate kinase compare
Pf6N2E2_4541 +0.5 0.9 hypothetical protein compare
Pf6N2E2_804 +0.5 2.4 Fructokinase (EC 2.7.1.4) compare
Pf6N2E2_4579 +0.5 1.2 Ribosomal RNA small subunit methyltransferase D (EC 2.1.1.-) compare
Pf6N2E2_4279 +0.5 3.3 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf6N2E2_4414 +0.5 1.3 FIG00953808: hypothetical protein compare
Pf6N2E2_3899 +0.5 2.9 Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) compare
Pf6N2E2_3799 +0.5 1.5 Histidine ammonia-lyase (EC 4.3.1.3) (from data) compare
Pf6N2E2_2017 +0.5 1.3 DNA-binding protein compare
Pf6N2E2_1775 +0.5 1.7 Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61) compare
Pf6N2E2_1009 +0.5 1.1 Inositol transport system permease protein compare
Pf6N2E2_5676 +0.5 1.6 Aspartokinase (EC 2.7.2.4) compare
Pf6N2E2_1614 +0.5 1.9 Transcriptional regulator, LysR family compare
Pf6N2E2_3655 +0.5 3.5 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf6N2E2_2483 +0.5 2.0 Deoxyribonuclease TatD compare
Pf6N2E2_1325 +0.5 1.6 FIG00954773: hypothetical protein compare
Pf6N2E2_4139 +0.5 3.6 Integral membrane protein TerC compare
Pf6N2E2_441 +0.5 1.6 FIG00958773: hypothetical protein compare
Pf6N2E2_1435 +0.5 1.3 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases; sulfonate monooxygenase compare
Pf6N2E2_2286 +0.5 1.5 FIG00953287: hypothetical protein compare
Pf6N2E2_2507 +0.5 2.2 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_1916 +0.5 1.3 Transcriptional regulator, LysR family compare
Pf6N2E2_748 +0.5 1.8 Plant-induced nitrilase (EC 3.5.5.1), hydrolyses beta-cyano-L-alanine compare
Pf6N2E2_5761 +0.4 1.3 Transcriptional regulator, TetR family compare
Pf6N2E2_5073 +0.4 1.1 Large-conductance mechanosensitive channel compare
Pf6N2E2_4489 +0.4 1.8 Putative activity regulator of membrane protease YbbK compare
Pf6N2E2_1588 +0.4 2.2 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf6N2E2_946 +0.4 1.4 Transcriptional regulator, LysR family compare
Pf6N2E2_2722 +0.4 1.6 FIG00954807: hypothetical protein compare
Pf6N2E2_1670 +0.4 1.7 conserved membrane protein compare
Pf6N2E2_5618 +0.4 2.9 Probable thiol oxidoreductase with 2 cytochrome c heme-binding sites compare
Pf6N2E2_41 +0.4 2.6 Two-component system response regulator QseB compare
Pf6N2E2_2416 +0.4 2.3 FIG00953472: hypothetical protein compare
Pf6N2E2_3362 +0.4 1.6 hypothetical protein compare
Pf6N2E2_846 +0.4 2.4 Universal stress protein family 5 compare
Pf6N2E2_1880 +0.4 2.3 Putative protein-S-isoprenylcysteine methyltransferase compare
Pf6N2E2_2012 +0.4 1.0 hypothetical protein compare
Pf6N2E2_1821 +0.4 1.0 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_1085 +0.4 0.9 Fe2+/Zn2+ uptake regulation proteins compare
Pf6N2E2_3557 +0.4 1.4 Chaperone-modulator protein CbpM compare
Pf6N2E2_1829 +0.4 2.0 Mesaconyl-CoA C1-C4 CoA transferase compare
Pf6N2E2_4167 +0.4 2.5 Zinc ABC transporter, ATP-binding protein ZnuC compare
Pf6N2E2_4596 +0.4 2.9 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_3283 +0.4 2.3 Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-) compare
Pf6N2E2_3956 +0.4 1.3 FIG00960943: hypothetical protein compare
Pf6N2E2_5405 +0.4 2.0 ABC transporter for D-Alanine, ATPase component (from data) compare
Pf6N2E2_5209 +0.4 1.9 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) compare
Pf6N2E2_6031 +0.4 1.3 hypothetical protein compare
Pf6N2E2_3008 +0.4 1.6 FIG00954242: hypothetical protein compare
Pf6N2E2_260 +0.4 1.8 Regulator of cell morphogenesis and NO signaling compare
Pf6N2E2_1959 +0.4 1.1 D-sorbitol dehydrogenase (EC 1.1.1.14) (from data) compare
Pf6N2E2_2352 +0.4 1.0 Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ) compare
Pf6N2E2_5156 +0.4 1.3 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_5461 +0.4 2.0 Ferredoxin-dependent glutamate synthase (EC 1.4.7.1) compare
Pf6N2E2_1156 +0.4 1.4 hypothetical protein compare
Pf6N2E2_648 +0.4 0.7 General secretion pathway protein K compare
Pf6N2E2_5667 +0.4 2.2 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_1329 +0.4 1.5 Phenylacetaldehyde dehydrogenase (EC 1.2.1.39) compare
Pf6N2E2_1320 +0.4 1.6 Putative response regulator compare
Pf6N2E2_2674 +0.4 0.5 FIG00953856: hypothetical protein compare
Pf6N2E2_699 +0.4 1.7 Heme d1 biosynthesis protein NirG compare
Pf6N2E2_2510 +0.4 1.7 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_2324 +0.4 1.4 Adenine phosphoribosyltransferase (EC 2.4.2.7) compare
Pf6N2E2_2682 +0.4 0.9 Transcriptional regulator, LysR family compare
Pf6N2E2_2353 +0.4 1.9 cell invasion protein - cytoplasmic compare
Pf6N2E2_1602 +0.4 1.5 hypothetical protein compare
Pf6N2E2_3944 +0.4 1.8 Protein of unknown function DUF81 compare
Pf6N2E2_3743 +0.4 1.8 General secretion pathway protein G compare
Pf6N2E2_1442 +0.4 1.9 Transcriptional regulators of sugar metabolism compare
Pf6N2E2_504 +0.4 1.4 DNA-binding response regulator compare
Pf6N2E2_2313 +0.4 1.0 Cytochrome c oxidase subunit CcoP (EC 1.9.3.1) compare
Pf6N2E2_4059 +0.4 0.6 DNA-binding protein HU-alpha compare
Pf6N2E2_114 +0.4 1.3 Polysaccharide biosynthesis protein compare
Pf6N2E2_3397 +0.4 0.8 Phage protein compare
Pf6N2E2_1671 +0.4 1.7 Transcriptional regulator, TetR family compare
Pf6N2E2_4332 +0.4 1.6 hypothetical protein compare
Pf6N2E2_5509 +0.4 1.4 FIG00955586: hypothetical protein compare
Pf6N2E2_84 +0.4 1.1 hypothetical protein compare
Pf6N2E2_5987 +0.4 2.2 OmpA family protein compare
Pf6N2E2_1438 +0.4 1.4 Methyltransferase (EC 2.1.1.-) compare


Specific Phenotypes

For 2 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Nickel (II) chloride hexahydrate across organisms