Experiment set7IT013 for Bacteroides thetaiotaomicron VPI-5482
Varel_Bryant medium with Thioridazine hydrochloride 0.00625 mM
Group: stressMedia: Varel_Bryant_medium_Glucose + Thioridazine hydrochloride (0.00625 mM) + Dimethyl Sulfoxide (0.0625 vol%)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Adam on 5/2/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: 2028 D5
Specific Phenotypes
For 1 genes in this experiment
For stress Thioridazine hydrochloride in Bacteroides thetaiotaomicron VPI-5482
For stress Thioridazine hydrochloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Carboxysome | 1 |
| Respiratory dehydrogenases 1 | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NADH to cytochrome bd oxidase electron transfer I | 2 | 1 | 1 |
| NADH to cytochrome bo oxidase electron transfer I | 2 | 1 | 1 |
| aerobic respiration III (alternative oxidase pathway) | 3 | 2 | 1 |
| aerobic respiration I (cytochrome c) | 4 | 2 | 1 |
| NAD(P)/NADPH interconversion | 6 | 2 | 1 |
| Fe(II) oxidation | 6 | 1 | 1 |