L-Proline carbon source
Group:
carbon source
Media:
M9 minimal media_noCarbon +
L-Proline (34.74333362 mM)
Culturing: SyringaeB728a_ML2, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler Helmann on
10/29/18
Media components: 2 mM
Magnesium sulfate, 0.1 mM
Calcium chloride, 12.8 g/L
Sodium phosphate dibasic heptahydrate, 3 g/L
Potassium phosphate monobasic, 0.5 g/L
Sodium Chloride, 1 g/L
Ammonium chloride
Specific Phenotypes
For 11 genes in this experiment
For carbon source L-Proline in Pseudomonas syringae pv. syringae B728a
For carbon source L-Proline across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|
| proline to cytochrome bo oxidase electron transfer | 2 | 2 | 1 |
| spermidine biosynthesis I | 2 | 2 | 1 |
| D-mannose degradation II | 2 | 1 | 1 |
| D-mannose degradation I | 2 | 1 | 1 |
| spermine biosynthesis | 2 | 1 | 1 |
| aminopropylcadaverine biosynthesis | 3 | 3 | 1 |
| L-arginine degradation I (arginase pathway) | 3 | 3 | 1 |
| β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation | 3 | 2 | 1 |
| L-proline degradation I | 3 | 2 | 1 |
| mannitol biosynthesis | 3 | 1 | 1 |
| GDP-mannose biosynthesis | 4 | 4 | 1 |
| mannitol degradation II | 4 | 2 | 1 |
| spermidine biosynthesis III | 4 | 2 | 1 |
| putrescine degradation II | 4 | 1 | 1 |
| ethene biosynthesis II (microbes) | 4 | 1 | 1 |
| 1,5-anhydrofructose degradation | 5 | 2 | 1 |
| folate transformations I | 13 | 9 | 2 |
| β-(1,4)-mannan degradation | 7 | 2 | 1 |
| superpathway of polyamine biosynthesis II | 8 | 7 | 1 |
| superpathway of polyamine biosynthesis I | 8 | 7 | 1 |
| superpathway of ornithine degradation | 8 | 4 | 1 |
| folate transformations III (E. coli) | 9 | 9 | 1 |
| glycolysis V (Pyrococcus) | 10 | 7 | 1 |
| reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) | 10 | 3 | 1 |
| folate transformations II (plants) | 11 | 10 | 1 |
| L-methionine salvage cycle III | 11 | 10 | 1 |
| glycolysis II (from fructose 6-phosphate) | 11 | 9 | 1 |
| colanic acid building blocks biosynthesis | 11 | 9 | 1 |
| superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation | 11 | 5 | 1 |
| L-methionine salvage cycle I (bacteria and plants) | 12 | 9 | 1 |
| gluconeogenesis I | 13 | 13 | 1 |
| glycolysis I (from glucose 6-phosphate) | 13 | 10 | 1 |
| superpathway of L-arginine and L-ornithine degradation | 13 | 7 | 1 |
| superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis | 14 | 7 | 1 |
| superpathway of arginine and polyamine biosynthesis | 17 | 16 | 1 |
| superpathway of glycolysis and the Entner-Doudoroff pathway | 17 | 14 | 1 |
| superpathway of hexitol degradation (bacteria) | 18 | 12 | 1 |
| gluconeogenesis II (Methanobacterium thermoautotrophicum) | 18 | 9 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 14 | 1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 13 | 1 |
| superpathway of N-acetylneuraminate degradation | 22 | 12 | 1 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 23 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 21 | 2 |