Experiment set7IT010 for Bacteroides thetaiotaomicron VPI-5482

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Varel_Bryant medium with Irgasan 0.005 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Irgasan (0.005 mM) + Dimethyl Sulfoxide (0.05 vol%)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Adam on 5/2/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: 2028 D1

Specific Phenotypes

For 3 genes in this experiment

For stress Irgasan in Bacteroides thetaiotaomicron VPI-5482

For stress Irgasan across organisms

SEED Subsystems

Subsystem #Specific
Protein chaperones 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.