Experiment set7IT007 for Pseudomonas syringae pv. syringae B728a

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Citric Acid carbon source

Group: carbon source
Media: M9 minimal media_noCarbon + Citric Acid (20.82032063 mM)
Culturing: SyringaeB728a_ML2, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler Helmann on 10/29/18
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 30 genes in this experiment

For carbon source Citric Acid in Pseudomonas syringae pv. syringae B728a

For carbon source Citric Acid across organisms

SEED Subsystems

Subsystem #Specific
Entner-Doudoroff Pathway 2
Glycolysis and Gluconeogenesis 2
Ribosome biogenesis bacterial 2
ABC transporter oligopeptide (TC 3.A.1.5.1) 1
Ammonia assimilation 1
Beta-Glucoside Metabolism 1
Biotin biosynthesis 1
Chorismate Synthesis 1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) 1
DNA-binding regulatory proteins, strays 1
Di-Inositol-Phosphate biosynthesis 1
Fructose and Mannose Inducible PTS 1
Fructose utilization 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Inositol catabolism 1
Lipid A modifications 1
Maltose and Maltodextrin Utilization 1
Mannitol Utilization 1
Polyamine Metabolism 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
Trehalose Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamate biosynthesis I 2 2 2
L-glutamine degradation II 1 1 1
L-glutamine degradation I 1 1 1
D-serine degradation 3 3 2
ammonia assimilation cycle III 3 3 2
L-serine degradation 3 3 2
L-cysteine degradation II 3 2 2
neolinustatin bioactivation 3 2 2
L-tryptophan degradation II (via pyruvate) 3 2 2
spermidine biosynthesis I 2 2 1
linustatin bioactivation 4 2 2
pseudouridine degradation 2 1 1
lotaustralin degradation 2 1 1
trehalose degradation II (cytosolic) 2 1 1
linamarin degradation 2 1 1
spermine biosynthesis 2 1 1
trehalose degradation I (low osmolarity) 2 1 1
myo-inositol biosynthesis 2 1 1
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis 5 2 2
aminopropylcadaverine biosynthesis 3 3 1
L-methionine biosynthesis II 6 5 2
GDP-α-D-glucose biosynthesis 3 2 1
trehalose degradation V 3 2 1
cellulose degradation II (fungi) 3 1 1
trehalose degradation IV 3 1 1
D-myo-inositol (1,4,5)-trisphosphate degradation 3 1 1
glycine betaine degradation III 7 7 2
L-glutamate and L-glutamine biosynthesis 7 5 2
L-asparagine biosynthesis III (tRNA-dependent) 4 4 1
sucrose degradation III (sucrose invertase) 4 4 1
glycine betaine degradation I 8 6 2
glutaminyl-tRNAgln biosynthesis via transamidation 4 3 1
L-mimosine degradation 8 4 2
spermidine biosynthesis III 4 2 1
glutathione-mediated detoxification I 8 3 2
putrescine degradation II 4 1 1
chorismate biosynthesis from 3-dehydroquinate 5 5 1
glucose and glucose-1-phosphate degradation 5 5 1
coumarin biosynthesis (via 2-coumarate) 5 2 1
glycolysis III (from glucose) 11 9 2
glycogen degradation II 6 5 1
homolactic fermentation 12 9 2
UDP-N-acetyl-D-glucosamine biosynthesis II 6 4 1
α-tomatine degradation 6 1 1
superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism 6 1 1
chorismate biosynthesis I 7 7 1
anaerobic energy metabolism (invertebrates, cytosol) 7 5 1
incomplete reductive TCA cycle 7 4 1
UDP-N-acetyl-D-galactosamine biosynthesis II 7 4 1
phytate degradation I 14 3 2
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 2
Bifidobacterium shunt 15 12 2
L-citrulline biosynthesis 8 7 1
superpathway of polyamine biosynthesis II 8 7 1
superpathway of polyamine biosynthesis I 8 7 1
glycogen degradation I 8 6 1
sucrose biosynthesis II 8 6 1
superpathway of ornithine degradation 8 4 1
Entner-Doudoroff pathway I 9 8 1
Entner-Doudoroff pathway III (semi-phosphorylative) 9 7 1
heterolactic fermentation 18 12 2
Entner-Doudoroff pathway II (non-phosphorylative) 9 6 1
chitin biosynthesis 9 5 1
1,3-propanediol biosynthesis (engineered) 9 4 1
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 10 1
Rubisco shunt 10 8 1
glycolysis V (Pyrococcus) 10 7 1
glycolysis IV 10 7 1
L-methionine salvage cycle III 11 10 1
glycolysis II (from fructose 6-phosphate) 11 9 1
glycolysis VI (from fructose) 11 7 1
reductive TCA cycle I 11 7 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 5 1
superpathway of L-citrulline metabolism 12 9 1
L-methionine salvage cycle I (bacteria and plants) 12 9 1
gluconeogenesis III 12 9 1
purine nucleobases degradation II (anaerobic) 24 16 2
chorismate biosynthesis II (archaea) 12 8 1
superpathway of L-tryptophan biosynthesis 13 13 1
glycolysis I (from glucose 6-phosphate) 13 10 1
superpathway of L-arginine and L-ornithine degradation 13 7 1
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 5 1
firefly bioluminescence 14 2 1
mixed acid fermentation 16 11 1
glycerol degradation to butanol 16 11 1
superpathway of arginine and polyamine biosynthesis 17 16 1
superpathway of glucose and xylose degradation 17 15 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 14 1
superpathway of anaerobic energy metabolism (invertebrates) 17 11 1
superpathway of aromatic amino acid biosynthesis 18 18 1
superpathway of hexitol degradation (bacteria) 18 12 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
superpathway of anaerobic sucrose degradation 19 14 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 13 1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle 22 18 1
superpathway of N-acetylneuraminate degradation 22 12 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 23 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 19 1
1-butanol autotrophic biosynthesis (engineered) 27 19 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 1
superpathway of chorismate metabolism 59 42 1