Culturing: SyringaeB728a_ML2, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
| Pathway | #Steps | #Present | #Specific |
| L-glutamate biosynthesis I | 2 | 2 | 2 |
| L-glutamine degradation II | 1 | 1 | 1 |
| L-glutamine degradation I | 1 | 1 | 1 |
| D-serine degradation | 3 | 3 | 2 |
| ammonia assimilation cycle III | 3 | 3 | 2 |
| L-serine degradation | 3 | 3 | 2 |
| L-tryptophan degradation II (via pyruvate) | 3 | 2 | 2 |
| neolinustatin bioactivation | 3 | 2 | 2 |
| L-cysteine degradation II | 3 | 2 | 2 |
| spermidine biosynthesis I | 2 | 2 | 1 |
| linustatin bioactivation | 4 | 2 | 2 |
| trehalose degradation II (cytosolic) | 2 | 1 | 1 |
| spermine biosynthesis | 2 | 1 | 1 |
| lotaustralin degradation | 2 | 1 | 1 |
| linamarin degradation | 2 | 1 | 1 |
| pseudouridine degradation | 2 | 1 | 1 |
| trehalose degradation I (low osmolarity) | 2 | 1 | 1 |
| myo-inositol biosynthesis | 2 | 1 | 1 |
| felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis | 5 | 2 | 2 |
| aminopropylcadaverine biosynthesis | 3 | 3 | 1 |
| L-methionine biosynthesis II | 6 | 5 | 2 |
| GDP-α-D-glucose biosynthesis | 3 | 2 | 1 |
| trehalose degradation V | 3 | 2 | 1 |
| D-myo-inositol (1,4,5)-trisphosphate degradation | 3 | 1 | 1 |
| trehalose degradation IV | 3 | 1 | 1 |
| cellulose degradation II (fungi) | 3 | 1 | 1 |
| glycine betaine degradation III | 7 | 7 | 2 |
| L-glutamate and L-glutamine biosynthesis | 7 | 5 | 2 |
| L-asparagine biosynthesis III (tRNA-dependent) | 4 | 4 | 1 |
| sucrose degradation III (sucrose invertase) | 4 | 4 | 1 |
| glycine betaine degradation I | 8 | 6 | 2 |
| glutaminyl-tRNAgln biosynthesis via transamidation | 4 | 3 | 1 |
| L-mimosine degradation | 8 | 4 | 2 |
| spermidine biosynthesis III | 4 | 2 | 1 |
| glutathione-mediated detoxification I | 8 | 3 | 2 |
| putrescine degradation II | 4 | 1 | 1 |
| glucose and glucose-1-phosphate degradation | 5 | 5 | 1 |
| chorismate biosynthesis from 3-dehydroquinate | 5 | 5 | 1 |
| coumarin biosynthesis (via 2-coumarate) | 5 | 2 | 1 |
| glycolysis III (from glucose) | 11 | 9 | 2 |
| glycogen degradation II | 6 | 5 | 1 |
| homolactic fermentation | 12 | 9 | 2 |
| UDP-N-acetyl-D-glucosamine biosynthesis II | 6 | 4 | 1 |
| α-tomatine degradation | 6 | 1 | 1 |
| superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism | 6 | 1 | 1 |
| chorismate biosynthesis I | 7 | 7 | 1 |
| anaerobic energy metabolism (invertebrates, cytosol) | 7 | 5 | 1 |
| UDP-N-acetyl-D-galactosamine biosynthesis II | 7 | 4 | 1 |
| incomplete reductive TCA cycle | 7 | 4 | 1 |
| phytate degradation I | 14 | 3 | 2 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 13 | 2 |
| Bifidobacterium shunt | 15 | 12 | 2 |
| L-citrulline biosynthesis | 8 | 7 | 1 |
| superpathway of polyamine biosynthesis I | 8 | 7 | 1 |
| superpathway of polyamine biosynthesis II | 8 | 7 | 1 |
| sucrose biosynthesis II | 8 | 6 | 1 |
| glycogen degradation I | 8 | 6 | 1 |
| superpathway of ornithine degradation | 8 | 4 | 1 |
| Entner-Doudoroff pathway I | 9 | 8 | 1 |
| Entner-Doudoroff pathway III (semi-phosphorylative) | 9 | 7 | 1 |
| heterolactic fermentation | 18 | 12 | 2 |
| Entner-Doudoroff pathway II (non-phosphorylative) | 9 | 6 | 1 |
| chitin biosynthesis | 9 | 5 | 1 |
| 1,3-propanediol biosynthesis (engineered) | 9 | 4 | 1 |
| superpathway of L-phenylalanine biosynthesis | 10 | 10 | 1 |
| superpathway of L-tyrosine biosynthesis | 10 | 10 | 1 |
| Rubisco shunt | 10 | 8 | 1 |
| glycolysis V (Pyrococcus) | 10 | 7 | 1 |
| glycolysis IV | 10 | 7 | 1 |
| L-methionine salvage cycle III | 11 | 10 | 1 |
| glycolysis II (from fructose 6-phosphate) | 11 | 9 | 1 |
| glycolysis VI (from fructose) | 11 | 7 | 1 |
| reductive TCA cycle I | 11 | 7 | 1 |
| superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation | 11 | 5 | 1 |
| L-methionine salvage cycle I (bacteria and plants) | 12 | 9 | 1 |
| gluconeogenesis III | 12 | 9 | 1 |
| superpathway of L-citrulline metabolism | 12 | 9 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 16 | 2 |
| chorismate biosynthesis II (archaea) | 12 | 8 | 1 |
| superpathway of L-tryptophan biosynthesis | 13 | 13 | 1 |
| glycolysis I (from glucose 6-phosphate) | 13 | 10 | 1 |
| superpathway of L-arginine and L-ornithine degradation | 13 | 7 | 1 |
| (S)-lactate fermentation to propanoate, acetate and hydrogen | 13 | 5 | 1 |
| firefly bioluminescence | 14 | 2 | 1 |
| glycerol degradation to butanol | 16 | 11 | 1 |
| mixed acid fermentation | 16 | 11 | 1 |
| superpathway of arginine and polyamine biosynthesis | 17 | 16 | 1 |
| superpathway of glucose and xylose degradation | 17 | 15 | 1 |
| superpathway of glycolysis and the Entner-Doudoroff pathway | 17 | 14 | 1 |
| superpathway of anaerobic energy metabolism (invertebrates) | 17 | 11 | 1 |
| superpathway of aromatic amino acid biosynthesis | 18 | 18 | 1 |
| superpathway of hexitol degradation (bacteria) | 18 | 12 | 1 |
| gluconeogenesis II (Methanobacterium thermoautotrophicum) | 18 | 9 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 14 | 1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 13 | 1 |
| superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle | 22 | 18 | 1 |
| superpathway of N-acetylneuraminate degradation | 22 | 12 | 1 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 23 | 1 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 19 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 19 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 21 | 1 |
| superpathway of chorismate metabolism | 59 | 42 | 1 |