Experiment set7IT005 for Desulfovibrio vulgaris Hildenborough JW710

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MoYLS4 with Tungstate 4mM

Group: stress
Media: MoYLS4 + Sodium tungstate dihydrate (4 mM), pH=7.2
Culturing: DvH_JW710, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Valentine on 2/10/2017
Media components: 1 g/L Yeast Extract, 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, 15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate, Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 23 genes in this experiment

For stress Sodium tungstate dihydrate in Desulfovibrio vulgaris Hildenborough JW710

For stress Sodium tungstate dihydrate across organisms

SEED Subsystems

Subsystem #Specific
Anaerobic respiratory reductases 2
Glycerol and Glycerol-3-phosphate Uptake and Utilization 2
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 2
Phosphate metabolism 2
Respiratory dehydrogenases 1 2
ABC transporter alkylphosphonate (TC 3.A.1.9.1) 1
H2:CoM-S-S-HTP oxidoreductase 1
High affinity phosphate transporter and control of PHO regulon 1
Hydantoin metabolism 1
Lactate utilization 1
Membrane-bound Ni, Fe-hydrogenase 1
Methanogenesis 1
Molybdenum cofactor biosynthesis 1
Transcription factors bacterial 1
Transport of Molybdenum 1
cAMP signaling in bacteria 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
glycerol-3-phosphate shuttle 2 2 1
glycerol-3-phosphate to fumarate electron transfer 2 2 1
glycerophosphodiester degradation 2 2 1
nitrate reduction IX (dissimilatory) 2 1 1
glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer 2 1 1
glycerol-3-phosphate to hydrogen peroxide electron transport 2 1 1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer 2 1 1
glycerol degradation I 3 2 1
sn-glycerol 3-phosphate anaerobic respiration 3 1 1
glycerol and glycerophosphodiester degradation 4 3 1
coenzyme B/coenzyme M regeneration I (methanophenazine-dependent) 4 1 1
creatinine degradation II 5 1 1