Experiment set7IT005 for Caulobacter crescentus NA1000

PYE 0.5x 4C

Group: temperature
Media: PYE (0.5x)
Culturing: Caulo_ML2, tube, Aerobic, at 4 (C), shaken=25 rpm
By: Sneha on 8-Aug-18
Media components: 1 g/L Bacto Peptone, 0.5 g/L Yeast Extract, 0.4 mM Magnesium sulfate, 0.25 mM Calcium chloride (final concentrations)

Specific Phenotypes

For 2 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Lysine Biosynthesis DAP Pathway	1
Threonine and Homoserine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• Lysine biosynthesis
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-homoserine biosynthesis	3	3	1
dipicolinate biosynthesis	4	3	1
spermidine biosynthesis II	4	2	1
L-methionine biosynthesis IV	4	2	1
ectoine biosynthesis	5	2	1
superpathway of L-threonine biosynthesis	6	6	1
norspermidine biosynthesis	6	2	1
L-lysine biosynthesis VI	7	6	1
L-lysine biosynthesis III	7	6	1
3-dehydroquinate biosynthesis II (archaea)	7	3	1
cremeomycin biosynthesis	7	2	1
superpathway of L-homoserine and L-methionine biosynthesis	8	6	1
grixazone biosynthesis	8	2	1
superpathway of polyamine biosynthesis III	8	2	1
L-lysine biosynthesis I	9	9	1
superpathway of S-adenosyl-L-methionine biosynthesis	9	7	1
superpathway of L-methionine biosynthesis (transsulfuration)	9	7	1
L-lysine biosynthesis II	9	7	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	12	1
chorismate biosynthesis II (archaea)	12	8	1
superpathway of L-isoleucine biosynthesis I	13	13	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	16	1
aspartate superpathway	25	23	1
platensimycin biosynthesis	26	6	1