Experiment set7IT004 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Manganese (II) chloride tetrahydrate 0.00078125 M

Group: stress
Media: LB + Manganese (II) chloride tetrahydrate (0.00078125 M)
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Hans_Hualan on 2/1/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 4 genes in this experiment

For stress Manganese (II) chloride tetrahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Manganese (II) chloride tetrahydrate across organisms

SEED Subsystems

Subsystem #Specific
Alanine biosynthesis 1
Cobalt-zinc-cadmium resistance 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-alanine biosynthesis III 1 1 1
L-cysteine degradation IV 1 1 1
cytidylyl molybdenum cofactor sulfurylation 2 1 1
bis(guanylyl molybdopterin) cofactor sulfurylation 3 1 1
thiazole component of thiamine diphosphate biosynthesis II 7 4 2
superpathway of L-alanine biosynthesis 4 4 1
tRNA-uridine 2-thiolation (mammalian mitochondria) 4 1 1
tRNA-uridine 2-thiolation (yeast mitochondria) 4 1 1
[2Fe-2S] iron-sulfur cluster biosynthesis 10 4 2
tRNA-uridine 2-thiolation (thermophilic bacteria) 5 1 1
superpathway of thiamine diphosphate biosynthesis II 11 8 2
molybdopterin biosynthesis 6 4 1
thiazole component of thiamine diphosphate biosynthesis I 6 3 1
tRNA-uridine 2-thiolation (cytoplasmic) 8 1 1
superpathway of thiamine diphosphate biosynthesis I 10 7 1
tRNA-uridine 2-thiolation and selenation (bacteria) 11 4 1