Experiment set7IT004 for Dechlorosoma suillum PS

Compare to:

anaerobic ALP with 3 all carbon sources and 5mM perchlorate

Group: anaerobic
Media: ALP_defined + Sodium perchlorate monohydrate (5 mM), pH=7.2
Culturing: PS_ML1, serum bottle, Anaerobic, at 30 (C)
Growth: about 2.8 generations
By: Kelly on 7/20/2018
Media components: 0.49 g/L Sodium phosphate monobasic monohydrate, 0.97 g/L Potassium phosphate dibasic, 0.1 g/L Potassium Chloride, 0.25 g/L Ammonium chloride, 40 mM Sodium D,L-Lactate, 1.1 g/L Sodium pyruvate, 0.8203 g/L Sodium acetate, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 2 genes in this experiment

For anaerobic Sodium perchlorate monohydrate in Dechlorosoma suillum PS

For anaerobic Sodium perchlorate monohydrate across organisms

SEED Subsystems

Subsystem #Specific
Arginine and Ornithine Degradation 1
Glutamate dehydrogenases 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Proline Synthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamate biosynthesis III 1 1 1
L-glutamate and L-glutamine biosynthesis 7 6 1