Experiment set7IT004 for Desulfovibrio vulgaris Hildenborough JW710

Compare to:

MoYLS4 with Tungstate 4mM

Group: stress
Media: MoYLS4 + Sodium tungstate dihydrate (4 mM), pH=7.2
Culturing: DvH_JW710, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Valentine on 2/10/2017
Media components: 1 g/L Yeast Extract, 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, 15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate, Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 23 genes in this experiment

For stress Sodium tungstate dihydrate in Desulfovibrio vulgaris Hildenborough JW710

For stress Sodium tungstate dihydrate across organisms

SEED Subsystems

Subsystem #Specific
Glycerol and Glycerol-3-phosphate Uptake and Utilization 2
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 2
Respiratory dehydrogenases 1 2
ABC transporter alkylphosphonate (TC 3.A.1.9.1) 1
Anaerobic respiratory reductases 1
H2:CoM-S-S-HTP oxidoreductase 1
Hydantoin metabolism 1
Lactate utilization 1
Methanogenesis 1
Molybdenum cofactor biosynthesis 1
Phosphate metabolism 1
Transport of Molybdenum 1
cAMP signaling in bacteria 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
coenzyme B/coenzyme M regeneration IV (H2-dependent) 1 1 1
glycerol-3-phosphate to fumarate electron transfer 2 2 1
glycerophosphodiester degradation 2 2 1
glycerol-3-phosphate shuttle 2 2 1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer 2 1 1
glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer 2 1 1
glycerol-3-phosphate to hydrogen peroxide electron transport 2 1 1
nitrate reduction IX (dissimilatory) 2 1 1
glycerol degradation I 3 2 1
sn-glycerol 3-phosphate anaerobic respiration 3 1 1
glycerol and glycerophosphodiester degradation 4 3 1
coenzyme B/coenzyme M regeneration I (methanophenazine-dependent) 4 1 1
creatinine degradation II 5 1 1
tRNA-uridine 2-thiolation and selenation (bacteria) 11 2 1