Experiment set7IT001 for Klebsiella michiganensis M5al

LB with Paraquat dichloride 0.1 mg/ml

Group: stress
Media: LB + Paraquat dichloride (0.1 mg/ml)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Apr-17
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1800 C2

Specific Phenotypes

For 17 genes in this experiment

For stress Paraquat dichloride in Klebsiella michiganensis M5al

For stress Paraquat dichloride across organisms

SEED Subsystems

Subsystem	#Specific
Cysteine Biosynthesis	3
Selenocysteine metabolism	2
ATP-dependent RNA helicases, bacterial	1
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more.	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Heat shock dnaK gene cluster extended	1
Oxidative stress	1
Queuosine-Archaeosine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Photosynthesis - antenna proteins
• Methionine metabolism
• Aminophosphonate metabolism
• Selenoamino acid metabolism
• High-mannose type N-glycan biosynthesis
• Nucleotide sugars metabolism
• Glycerophospholipid metabolism
• Sphingolipid metabolism
• Pyruvate metabolism
• Vitamin B6 metabolism
• Porphyrin and chlorophyll metabolism
• Aminoacyl-tRNA biosynthesis
• Biosynthesis of type II polyketide backbone
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-selenocysteine biosynthesis I (bacteria)	3	3	1
cardiolipin biosynthesis I	3	3	1
molybdenum cofactor biosynthesis	3	3	1
cardiolipin biosynthesis II	3	3	1
cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas)	4	3	1
superpathway of cardiolipin biosynthesis (bacteria)	13	10	2
superpathway of phospholipid biosynthesis III (E. coli)	12	11	1