Experiment set7H34 for Pseudomonas stutzeri RCH2

Compare to:

RCH2 defined media with Benzalkonium Chloride 0.002 mg/ml

Group: stress
Media: RCH2_defined_no_vitamin + Benzalkonium Chloride (0.002 mg/ml), pH=7.2
Culturing: psRCH2_ML7, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Adam on 8/13/2013
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM Sodium D,L-Lactate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate)
Growth plate: 644 E7,E8

Specific Phenotypes

For 74 genes in this experiment

For stress Benzalkonium Chloride in Pseudomonas stutzeri RCH2

For stress Benzalkonium Chloride across organisms

SEED Subsystems

Subsystem #Specific
Coenzyme A Biosynthesis 2
Coenzyme PQQ synthesis 2
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 2
Pyrroloquinoline Quinone biosynthesis 2
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 2
SigmaB stress responce regulation 2
ATP-dependent RNA helicases, bacterial 1
Alanine biosynthesis 1
Bacterial Chemotaxis 1
Cobalt-zinc-cadmium resistance 1
Entner-Doudoroff Pathway 1
Glutathione-dependent pathway of formaldehyde detoxification 1
Hemin transport system 1
Lactate utilization 1
Lysine Biosynthesis DAP Pathway 1
Methylglyoxal Metabolism 1
Multidrug Resistance Efflux Pumps 1
Peptidoglycan Biosynthesis 1
Proteolysis in bacteria, ATP-dependent 1
Respiratory dehydrogenases 1 1
Restriction-Modification System 1
Ribosome biogenesis bacterial 1
Soluble cytochromes and functionally related electron carriers 1
Ton and Tol transport systems 1
ZZ gjo need homes 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
ethanol degradation I 2 2 2
L-alanine biosynthesis III 1 1 1
L-cysteine degradation IV 1 1 1
β-alanine biosynthesis III 1 1 1
acetaldehyde biosynthesis I 1 1 1
acetate formation from acetyl-CoA (succinate) 1 1 1
ethanol degradation II 3 3 2
pyruvate fermentation to ethanol I 3 2 2
L-methionine degradation II 3 2 2
pyruvate fermentation to ethanol III 3 2 2
putrescine degradation V 2 2 1
L-threonine degradation IV 2 2 1
phytol degradation 4 3 2
pyruvate fermentation to ethanol II 2 1 1
cytidylyl molybdenum cofactor sulfurylation 2 1 1
phenylethylamine degradation I 2 1 1
ethylene glycol degradation 2 1 1
phenylethanol degradation 2 1 1
pseudouridine degradation 2 1 1
putrescine degradation I 2 1 1
phenylethylamine degradation II 2 1 1
ethanolamine utilization 5 5 2
acetylene degradation (anaerobic) 5 4 2
L-serine biosynthesis I 3 3 1
ethanol degradation IV 3 3 1
formaldehyde oxidation II (glutathione-dependent) 3 3 1
pyruvate fermentation to acetate V 3 3 1
L-threonine degradation I 6 5 2
L-phenylalanine degradation II (anaerobic) 3 2 1
pyruvate fermentation to acetate VI 3 2 1
L-leucine degradation III 3 2 1
L-valine degradation II 3 2 1
ethanol degradation III 3 2 1
L-isoleucine degradation II 3 2 1
putrescine degradation IV 3 2 1
hypotaurine degradation 3 2 1
2-hydroxypenta-2,4-dienoate degradation 3 1 1
bis(guanylyl molybdopterin) cofactor sulfurylation 3 1 1
L-methionine degradation III 3 1 1
2-deoxy-D-ribose degradation I 3 1 1
styrene degradation 3 1 1
sulfoacetaldehyde degradation IV 3 1 1
2-deoxy-α-D-ribose 1-phosphate degradation 3 1 1
2-aminoethylphosphonate degradation I 3 1 1
histamine degradation 3 1 1
noradrenaline and adrenaline degradation 13 4 4
L-isoleucine biosynthesis I (from threonine) 7 7 2
thiazole component of thiamine diphosphate biosynthesis II 7 5 2
pyrroloquinoline quinone biosynthesis 7 4 2
serotonin degradation 7 3 2
superpathway of L-serine and glycine biosynthesis I 4 4 1
superpathway of L-alanine biosynthesis 4 4 1
CDP-diacylglycerol biosynthesis I 4 4 1
phosphopantothenate biosynthesis I 4 4 1
CDP-diacylglycerol biosynthesis II 4 4 1
fatty acid α-oxidation I (plants) 4 2 1
L-tyrosine degradation III 4 2 1
L-phenylalanine degradation III 4 2 1
phosphopantothenate biosynthesis III (archaea) 4 2 1
tRNA-uridine 2-thiolation (yeast mitochondria) 4 1 1
putrescine degradation III 4 1 1
L-tryptophan degradation X (mammalian, via tryptamine) 4 1 1
salidroside biosynthesis 4 1 1
D-arabinose degradation II 4 1 1
tRNA-uridine 2-thiolation (mammalian mitochondria) 4 1 1
superpathway of coenzyme A biosynthesis I (bacteria) 9 9 2
superpathway of fermentation (Chlamydomonas reinhardtii) 9 5 2
pyruvate fermentation to isobutanol (engineered) 5 4 1
mitochondrial NADPH production (yeast) 5 3 1
CDP-diacylglycerol biosynthesis III 5 3 1
(S)-propane-1,2-diol degradation 5 3 1
protein S-nitrosylation and denitrosylation 5 3 1
octane oxidation 5 3 1
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
[2Fe-2S] iron-sulfur cluster biosynthesis 10 4 2
dopamine degradation 5 1 1
catechol degradation I (meta-cleavage pathway) 5 1 1
phenylethanol biosynthesis 5 1 1
tRNA-uridine 2-thiolation (thermophilic bacteria) 5 1 1
superpathway of thiamine diphosphate biosynthesis II 11 9 2
phosphatidylglycerol biosynthesis I 6 6 1
phosphatidylglycerol biosynthesis II 6 6 1
superpathway of L-threonine metabolism 18 13 3
molybdopterin biosynthesis 6 4 1
thiazole component of thiamine diphosphate biosynthesis I 6 4 1
3-methyl-branched fatty acid α-oxidation 6 3 1
superpathway of pyrimidine deoxyribonucleosides degradation 6 2 1
triethylamine degradation 6 1 1
alkane oxidation 6 1 1
superpathway of L-isoleucine biosynthesis I 13 13 2
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 6 2
3-methylbutanol biosynthesis (engineered) 7 6 1
superpathway of glycol metabolism and degradation 7 5 1
hypoglycin biosynthesis 14 4 2
ceramide degradation by α-oxidation 7 2 1
superpathway of purine deoxyribonucleosides degradation 7 2 1
succinate fermentation to butanoate 7 2 1
toluene degradation I (aerobic) (via o-cresol) 7 1 1
toluene degradation V (aerobic) (via toluene-cis-diol) 7 1 1
limonene degradation IV (anaerobic) 7 1 1
catechol degradation II (meta-cleavage pathway) 7 1 1
mixed acid fermentation 16 12 2
superpathway of ornithine degradation 8 6 1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 5 1
butanol and isobutanol biosynthesis (engineered) 8 3 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation 8 1 1
aromatic biogenic amine degradation (bacteria) 8 1 1
p-cumate degradation 8 1 1
tRNA-uridine 2-thiolation (cytoplasmic) 8 1 1
superpathway of branched chain amino acid biosynthesis 17 17 2
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 8 2
L-lysine biosynthesis I 9 9 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 1
TCA cycle VII (acetate-producers) 9 8 1
heterolactic fermentation 18 15 2
Entner-Doudoroff pathway II (non-phosphorylative) 9 5 1
L-phenylalanine degradation IV (mammalian, via side chain) 9 3 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 16 2
superpathway of coenzyme A biosynthesis II (plants) 10 9 1
superpathway of thiamine diphosphate biosynthesis I 10 8 1
anaerobic energy metabolism (invertebrates, mitochondrial) 10 5 1
meta cleavage pathway of aromatic compounds 10 3 1
superpathway of N-acetylneuraminate degradation 22 14 2
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 6 1
tRNA-uridine 2-thiolation and selenation (bacteria) 11 5 1
superpathway of phenylethylamine degradation 11 4 1
p-cymene degradation 11 1 1
superpathway of phospholipid biosynthesis III (E. coli) 12 10 1
superpathway of C1 compounds oxidation to CO2 12 4 1
L-tryptophan degradation XII (Geobacillus) 12 1 1
naphthalene degradation to acetyl-CoA 12 1 1
L-tryptophan degradation IX 12 1 1
superpathway of cardiolipin biosynthesis (bacteria) 13 9 1
superpathway of L-arginine and L-ornithine degradation 13 8 1
toluene degradation IV (aerobic) (via catechol) 13 3 1
L-tryptophan degradation V (side chain pathway) 13 1 1
superpathway of anaerobic energy metabolism (invertebrates) 17 9 1
type I lipoteichoic acid biosynthesis (S. aureus) 17 5 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 1
mandelate degradation to acetyl-CoA 18 9 1
superpathway of anaerobic sucrose degradation 19 15 1
aspartate superpathway 25 22 1
anaerobic aromatic compound degradation (Thauera aromatica) 27 3 1
superpathway of phospholipid biosynthesis II (plants) 28 10 1
superpathway of aerobic toluene degradation 30 7 1
superpathway of aromatic compound degradation via 3-oxoadipate 35 11 1
superpathway of pentose and pentitol degradation 42 7 1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate 42 5 1