Culturing: psRCH2_ML7, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
| Pathway | #Steps | #Present | #Specific |
| ethanol degradation I | 2 | 2 | 2 |
| L-cysteine degradation IV | 1 | 1 | 1 |
| L-alanine biosynthesis III | 1 | 1 | 1 |
| β-alanine biosynthesis III | 1 | 1 | 1 |
| acetaldehyde biosynthesis I | 1 | 1 | 1 |
| acetate formation from acetyl-CoA (succinate) | 1 | 1 | 1 |
| ethanol degradation II | 3 | 3 | 2 |
| L-methionine degradation II | 3 | 2 | 2 |
| pyruvate fermentation to ethanol III | 3 | 2 | 2 |
| pyruvate fermentation to ethanol I | 3 | 2 | 2 |
| putrescine degradation V | 2 | 2 | 1 |
| L-threonine degradation IV | 2 | 2 | 1 |
| phytol degradation | 4 | 3 | 2 |
| pseudouridine degradation | 2 | 1 | 1 |
| putrescine degradation I | 2 | 1 | 1 |
| ethylene glycol degradation | 2 | 1 | 1 |
| phenylethylamine degradation II | 2 | 1 | 1 |
| pyruvate fermentation to ethanol II | 2 | 1 | 1 |
| phenylethylamine degradation I | 2 | 1 | 1 |
| phenylethanol degradation | 2 | 1 | 1 |
| cytidylyl molybdenum cofactor sulfurylation | 2 | 1 | 1 |
| ethanolamine utilization | 5 | 5 | 2 |
| acetylene degradation (anaerobic) | 5 | 4 | 2 |
| L-serine biosynthesis I | 3 | 3 | 1 |
| formaldehyde oxidation II (glutathione-dependent) | 3 | 3 | 1 |
| ethanol degradation IV | 3 | 3 | 1 |
| pyruvate fermentation to acetate V | 3 | 3 | 1 |
| L-threonine degradation I | 6 | 5 | 2 |
| hypotaurine degradation | 3 | 2 | 1 |
| L-isoleucine degradation II | 3 | 2 | 1 |
| L-leucine degradation III | 3 | 2 | 1 |
| L-phenylalanine degradation II (anaerobic) | 3 | 2 | 1 |
| L-valine degradation II | 3 | 2 | 1 |
| ethanol degradation III | 3 | 2 | 1 |
| pyruvate fermentation to acetate VI | 3 | 2 | 1 |
| putrescine degradation IV | 3 | 2 | 1 |
| sulfoacetaldehyde degradation IV | 3 | 1 | 1 |
| histamine degradation | 3 | 1 | 1 |
| L-methionine degradation III | 3 | 1 | 1 |
| 2-deoxy-D-ribose degradation I | 3 | 1 | 1 |
| 2-deoxy-α-D-ribose 1-phosphate degradation | 3 | 1 | 1 |
| 2-hydroxypenta-2,4-dienoate degradation | 3 | 1 | 1 |
| 2-aminoethylphosphonate degradation I | 3 | 1 | 1 |
| bis(guanylyl molybdopterin) cofactor sulfurylation | 3 | 1 | 1 |
| styrene degradation | 3 | 1 | 1 |
| noradrenaline and adrenaline degradation | 13 | 4 | 4 |
| L-isoleucine biosynthesis I (from threonine) | 7 | 7 | 2 |
| thiazole component of thiamine diphosphate biosynthesis II | 7 | 5 | 2 |
| pyrroloquinoline quinone biosynthesis | 7 | 4 | 2 |
| serotonin degradation | 7 | 3 | 2 |
| superpathway of L-serine and glycine biosynthesis I | 4 | 4 | 1 |
| superpathway of L-alanine biosynthesis | 4 | 4 | 1 |
| phosphopantothenate biosynthesis I | 4 | 4 | 1 |
| CDP-diacylglycerol biosynthesis II | 4 | 4 | 1 |
| CDP-diacylglycerol biosynthesis I | 4 | 4 | 1 |
| L-tyrosine degradation III | 4 | 2 | 1 |
| phosphopantothenate biosynthesis III (archaea) | 4 | 2 | 1 |
| L-phenylalanine degradation III | 4 | 2 | 1 |
| fatty acid α-oxidation I (plants) | 4 | 2 | 1 |
| tRNA-uridine 2-thiolation (yeast mitochondria) | 4 | 1 | 1 |
| putrescine degradation III | 4 | 1 | 1 |
| tRNA-uridine 2-thiolation (mammalian mitochondria) | 4 | 1 | 1 |
| D-arabinose degradation II | 4 | 1 | 1 |
| salidroside biosynthesis | 4 | 1 | 1 |
| L-tryptophan degradation X (mammalian, via tryptamine) | 4 | 1 | 1 |
| superpathway of coenzyme A biosynthesis I (bacteria) | 9 | 9 | 2 |
| superpathway of fermentation (Chlamydomonas reinhardtii) | 9 | 5 | 2 |
| pyruvate fermentation to isobutanol (engineered) | 5 | 4 | 1 |
| (S)-propane-1,2-diol degradation | 5 | 3 | 1 |
| mitochondrial NADPH production (yeast) | 5 | 3 | 1 |
| octane oxidation | 5 | 3 | 1 |
| CDP-diacylglycerol biosynthesis III | 5 | 3 | 1 |
| protein S-nitrosylation and denitrosylation | 5 | 3 | 1 |
| sphingosine and sphingosine-1-phosphate metabolism | 10 | 4 | 2 |
| [2Fe-2S] iron-sulfur cluster biosynthesis | 10 | 4 | 2 |
| phenylethanol biosynthesis | 5 | 1 | 1 |
| dopamine degradation | 5 | 1 | 1 |
| catechol degradation I (meta-cleavage pathway) | 5 | 1 | 1 |
| tRNA-uridine 2-thiolation (thermophilic bacteria) | 5 | 1 | 1 |
| superpathway of thiamine diphosphate biosynthesis II | 11 | 9 | 2 |
| phosphatidylglycerol biosynthesis I | 6 | 6 | 1 |
| phosphatidylglycerol biosynthesis II | 6 | 6 | 1 |
| superpathway of L-threonine metabolism | 18 | 13 | 3 |
| molybdopterin biosynthesis | 6 | 4 | 1 |
| thiazole component of thiamine diphosphate biosynthesis I | 6 | 4 | 1 |
| 3-methyl-branched fatty acid α-oxidation | 6 | 3 | 1 |
| superpathway of pyrimidine deoxyribonucleosides degradation | 6 | 2 | 1 |
| alkane oxidation | 6 | 1 | 1 |
| triethylamine degradation | 6 | 1 | 1 |
| superpathway of L-isoleucine biosynthesis I | 13 | 13 | 2 |
| superpathway of Clostridium acetobutylicum solventogenic fermentation | 13 | 6 | 2 |
| 3-methylbutanol biosynthesis (engineered) | 7 | 6 | 1 |
| superpathway of glycol metabolism and degradation | 7 | 5 | 1 |
| hypoglycin biosynthesis | 14 | 4 | 2 |
| ceramide degradation by α-oxidation | 7 | 2 | 1 |
| succinate fermentation to butanoate | 7 | 2 | 1 |
| superpathway of purine deoxyribonucleosides degradation | 7 | 2 | 1 |
| limonene degradation IV (anaerobic) | 7 | 1 | 1 |
| toluene degradation I (aerobic) (via o-cresol) | 7 | 1 | 1 |
| toluene degradation V (aerobic) (via toluene-cis-diol) | 7 | 1 | 1 |
| catechol degradation II (meta-cleavage pathway) | 7 | 1 | 1 |
| mixed acid fermentation | 16 | 12 | 2 |
| superpathway of ornithine degradation | 8 | 6 | 1 |
| superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) | 8 | 5 | 1 |
| butanol and isobutanol biosynthesis (engineered) | 8 | 3 | 1 |
| ceramide and sphingolipid recycling and degradation (yeast) | 16 | 4 | 2 |
| tRNA-uridine 2-thiolation (cytoplasmic) | 8 | 1 | 1 |
| aromatic biogenic amine degradation (bacteria) | 8 | 1 | 1 |
| 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation | 8 | 1 | 1 |
| p-cumate degradation | 8 | 1 | 1 |
| superpathway of branched chain amino acid biosynthesis | 17 | 17 | 2 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 8 | 2 |
| L-lysine biosynthesis I | 9 | 9 | 1 |
| superpathway of sulfate assimilation and cysteine biosynthesis | 9 | 9 | 1 |
| TCA cycle VII (acetate-producers) | 9 | 8 | 1 |
| heterolactic fermentation | 18 | 15 | 2 |
| Entner-Doudoroff pathway II (non-phosphorylative) | 9 | 5 | 1 |
| L-phenylalanine degradation IV (mammalian, via side chain) | 9 | 3 | 1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 16 | 2 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 9 | 1 |
| superpathway of thiamine diphosphate biosynthesis I | 10 | 8 | 1 |
| anaerobic energy metabolism (invertebrates, mitochondrial) | 10 | 5 | 1 |
| meta cleavage pathway of aromatic compounds | 10 | 3 | 1 |
| superpathway of N-acetylneuraminate degradation | 22 | 14 | 2 |
| superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation | 11 | 6 | 1 |
| tRNA-uridine 2-thiolation and selenation (bacteria) | 11 | 5 | 1 |
| superpathway of phenylethylamine degradation | 11 | 4 | 1 |
| p-cymene degradation | 11 | 1 | 1 |
| superpathway of phospholipid biosynthesis III (E. coli) | 12 | 10 | 1 |
| superpathway of C1 compounds oxidation to CO2 | 12 | 4 | 1 |
| L-tryptophan degradation IX | 12 | 1 | 1 |
| L-tryptophan degradation XII (Geobacillus) | 12 | 1 | 1 |
| naphthalene degradation to acetyl-CoA | 12 | 1 | 1 |
| superpathway of cardiolipin biosynthesis (bacteria) | 13 | 9 | 1 |
| superpathway of L-arginine and L-ornithine degradation | 13 | 8 | 1 |
| toluene degradation IV (aerobic) (via catechol) | 13 | 3 | 1 |
| L-tryptophan degradation V (side chain pathway) | 13 | 1 | 1 |
| superpathway of anaerobic energy metabolism (invertebrates) | 17 | 9 | 1 |
| type I lipoteichoic acid biosynthesis (S. aureus) | 17 | 5 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis I | 18 | 16 | 1 |
| mandelate degradation to acetyl-CoA | 18 | 9 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 15 | 1 |
| aspartate superpathway | 25 | 22 | 1 |
| anaerobic aromatic compound degradation (Thauera aromatica) | 27 | 3 | 1 |
| superpathway of phospholipid biosynthesis II (plants) | 28 | 10 | 1 |
| superpathway of aerobic toluene degradation | 30 | 7 | 1 |
| superpathway of aromatic compound degradation via 3-oxoadipate | 35 | 11 | 1 |
| superpathway of pentose and pentitol degradation | 42 | 7 | 1 |
| superpathway of aromatic compound degradation via 2-hydroxypentadienoate | 42 | 5 | 1 |