Experiment set7H22 for Pseudomonas stutzeri RCH2
RCH2 defined media with Doxycycline hyclate 0.0006 mg/ml
Group: stressMedia: RCH2_defined_no_vitamin + Doxycycline hyclate (6e-04 mg/ml), pH=7.2
Culturing: psRCH2_ML7, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Adam on 8/12/2013
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM Sodium D,L-Lactate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate)
Specific Phenotypes
For 5 genes in this experiment
For stress Doxycycline hyclate in Pseudomonas stutzeri RCH2
For stress Doxycycline hyclate across organisms
Metabolic Maps
Color code by fitness: see overview map or list of maps.
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| UDP-N-acetyl-α-D-quinovosamine biosynthesis | 2 | 2 | 1 |
| UDP-2-acetamido-4-amino-2,4,6-trideoxy-α-D-galactose biosynthesis | 2 | 2 | 1 |
| UDP-N-acetyl-α-D-fucosamine biosynthesis | 2 | 1 | 1 |
| UDP-yelosamine biosynthesis | 3 | 2 | 1 |
| UDP-N,N'-diacetylbacillosamine biosynthesis | 3 | 1 | 1 |
| superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis | 24 | 9 | 1 |