Experiment set6S734 for Mycobacterium tuberculosis H37Rv
7H9 with Sisomicin sulfate salt 0.07 mM
Group: stressMedia: 7H9 + Sisomicin sulfate salt (0.07 mM)
Culturing: MycoTube_ML10, tube, Aerobic, at 37 (C)
By: Kayla Dinshaw on 3/7/25
Media components: 0.5 g/L Ammonium Sulfate, 0.5 g/L L-Glutamic acid, 0.1 g/L Trisodium citrate dihydrate, 0.001 g/L Pyridoxine HCl, 0.0005 g/L biotin, 2.5 g/L Disodium phosphate, 1 g/L Potassium phosphate monobasic, 0.04 g/L Ferric ammonium citrate, 0.05 g/L Magnesium sulfate, 0.0005 g/L Calcium chloride, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L copper (II) chloride dihydrate, 5 g/L bovine serum albumin, 2 g/L D-Glucose, 4.25 g/L Sodium Chloride, 0.5 vol% Tween 80
Specific Phenotypes
For 7 genes in this experiment
For stress Sisomicin sulfate salt in Mycobacterium tuberculosis H37Rv
For stress Sisomicin sulfate salt across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Peptidoglycan Biosynthesis | 1 |
| Type IV pilus | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 12 | 2 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 12 | 2 |
| peptidoglycan biosynthesis V (β-lactam resistance) | 17 | 11 | 2 |
| peptidoglycan biosynthesis I (meso-diaminopimelate containing) | 12 | 11 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 3 | 1 |
| peptidoglycan biosynthesis III (mycobacteria) | 15 | 14 | 1 |