Experiment set6S732 for Mycobacterium tuberculosis H37Rv

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7H9 with Sodium nitrate 4 mM

Group: stress
Media: 7H9 + Sodium nitrate (4 mM)
Culturing: MycoTube_ML10, tube, Aerobic, at 37 (C)
By: Kayla Dinshaw on 3/7/25
Media components: 0.5 g/L Ammonium Sulfate, 0.5 g/L L-Glutamic acid, 0.1 g/L Trisodium citrate dihydrate, 0.001 g/L Pyridoxine HCl, 0.0005 g/L biotin, 2.5 g/L Disodium phosphate, 1 g/L Potassium phosphate monobasic, 0.04 g/L Ferric ammonium citrate, 0.05 g/L Magnesium sulfate, 0.0005 g/L Calcium chloride, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L copper (II) chloride dihydrate, 5 g/L bovine serum albumin, 2 g/L D-Glucose, 4.25 g/L Sodium Chloride, 0.5 vol% Tween 80

Specific Phenotypes

For 4 genes in this experiment

For stress Sodium nitrate in Mycobacterium tuberculosis H37Rv

For stress Sodium nitrate across organisms

SEED Subsystems

Subsystem #Specific
Cysteine Biosynthesis 1
Glycine and Serine Utilization 1
Methionine Biosynthesis 1
Methionine Degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-cysteine biosynthesis III (from L-homocysteine) 2 2 1
hydrogen sulfide biosynthesis II (mammalian) 6 4 3
taurine biosynthesis III 3 1 1
homocysteine and cysteine interconversion 4 3 1
L-cysteine biosynthesis V (mycobacteria) 4 2 1
superpathway of L-cysteine biosynthesis (mammalian) 5 4 1
superpathway of L-cysteine biosynthesis (fungi) 6 5 1
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) 10 8 1
superpathway of L-methionine salvage and degradation 16 10 1