Experiment set6S730 for Mycobacterium tuberculosis H37Rv

7H9 with Sodium nitrite 5 mM

Group: stress
Media: 7H9 + Sodium nitrite (5 mM)
Culturing: MycoTube_ML10, tube, Aerobic, at 37 (C)
By: Kayla Dinshaw on 3/7/25
Media components: 0.5 g/L Ammonium Sulfate, 0.5 g/L L-Glutamic acid, 0.1 g/L Trisodium citrate dihydrate, 0.001 g/L Pyridoxine HCl, 0.0005 g/L biotin, 2.5 g/L Disodium phosphate, 1 g/L Potassium phosphate monobasic, 0.04 g/L Ferric ammonium citrate, 0.05 g/L Magnesium sulfate, 0.0005 g/L Calcium chloride, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L copper (II) chloride dihydrate, 5 g/L bovine serum albumin, 2 g/L D-Glucose, 4.25 g/L Sodium Chloride, 0.5 vol% Tween 80

Specific Phenotypes

For 3 genes in this experiment

For stress Sodium nitrite in Mycobacterium tuberculosis H37Rv

For stress Sodium nitrite across organisms

SEED Subsystems

Subsystem	#Specific
Cysteine Biosynthesis	2
Glycine and Serine Utilization	2
Methionine Biosynthesis	2
Methionine Degradation	2

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Methionine metabolism
• Selenoamino acid metabolism
• Glycine, serine and threonine metabolism
• Cysteine metabolism
• Nitrogen metabolism
• Sulfur metabolism
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-cysteine degradation II	3	3	3
L-cysteine biosynthesis III (from L-homocysteine)	2	2	2
homocysteine and cysteine interconversion	4	3	3
L-serine degradation	3	3	2
hydrogen sulfide biosynthesis II (mammalian)	6	4	4
L-tryptophan degradation II (via pyruvate)	3	2	2
L-methionine degradation II	3	2	2
D-serine degradation	3	2	2
L-methionine biosynthesis II	6	5	3
superpathway of L-cysteine biosynthesis (fungi)	6	5	3
superpathway of L-cysteine biosynthesis (mammalian)	5	4	2
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis	5	2	2
L-threonine degradation I	6	5	2
taurine biosynthesis III	3	1	1
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)	10	8	3
L-isoleucine biosynthesis I (from threonine)	7	7	2
glycine betaine degradation III	7	4	2
L-mimosine degradation	8	4	2
glycine betaine degradation I	8	4	2
L-cysteine biosynthesis V (mycobacteria)	4	2	1
glutathione-mediated detoxification I	8	2	2
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	3
L-methionine biosynthesis I	5	3	1
superpathway of L-isoleucine biosynthesis I	13	13	2
L-cysteine biosynthesis VI (reverse transsulfuration)	7	4	1
hypoglycin biosynthesis	14	4	2
superpathway of L-homoserine and L-methionine biosynthesis	8	6	1
superpathway of L-methionine salvage and degradation	16	10	2
superpathway of branched chain amino acid biosynthesis	17	17	2
superpathway of S-adenosyl-L-methionine biosynthesis	9	7	1
superpathway of L-methionine biosynthesis (transsulfuration)	9	7	1
superpathway of L-threonine metabolism	18	12	2
purine nucleobases degradation II (anaerobic)	24	12	2
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	16	1
aspartate superpathway	25	23	1