Experiment set6IT094 for Pseudomonas fluorescens FW300-N2C3

4C survival; 1152 hrs

Group: survival
Media: LB
Culturing: pseudo5_N2-C3_1_ML2, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 7.5 generations
By: Jayashree on 10/28/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 34 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Bacterial Chemotaxis	3
DNA repair, bacterial MutL-MutS system	2
Flagellar motility	2
Terminal cytochrome d ubiquinol oxidases	2
Terminal cytochrome oxidases	2
Two-component regulatory systems in Campylobacter	2
ABC transporter oligopeptide (TC 3.A.1.5.1)	1
Biogenesis of cbb3-type cytochrome c oxidases	1
Cobalt-zinc-cadmium resistance	1
Cysteine Biosynthesis	1
DNA-binding regulatory proteins, strays	1
Flagellum	1
Glycogen metabolism	1
Hydantoin metabolism	1
Methylcitrate cycle	1
Murein hydrolase regulation and cell death	1
Phenylpropanoid compound degradation	1
Propionate-CoA to Succinate Module	1
Terminal cytochrome C oxidases	1
Trehalose Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Starch and sucrose metabolism
• Propanoate metabolism
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Valine, leucine and isoleucine degradation
• Geraniol degradation
• Lysine degradation
• Tryptophan metabolism
• beta-Alanine metabolism
• alpha-Linolenic acid metabolism
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Limonene and pinene degradation
• Caprolactam degradation
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
trehalose biosynthesis IV	1	1	1
ferulate degradation	3	3	2
aerobic respiration III (alternative oxidase pathway)	3	3	1
benzoyl-CoA biosynthesis	3	3	1
capsaicin biosynthesis	7	3	2
glycogen biosynthesis I (from ADP-D-Glucose)	4	3	1
2-methyl-branched fatty acid β-oxidation	14	11	3
adipate degradation	5	5	1
2-methylcitrate cycle I	5	5	1
adipate biosynthesis	5	4	1
fatty acid β-oxidation IV (unsaturated, even number)	5	4	1
fatty acid β-oxidation II (plant peroxisome)	5	3	1
benzoate biosynthesis III (CoA-dependent, non-β-oxidative)	5	3	1
(8E,10E)-dodeca-8,10-dienol biosynthesis	11	6	2
2-methylcitrate cycle II	6	5	1
methyl ketone biosynthesis (engineered)	6	3	1
fatty acid β-oxidation I (generic)	7	5	1
fatty acid β-oxidation VI (mammalian peroxisome)	7	4	1
benzoyl-CoA degradation I (aerobic)	7	3	1
glycogen biosynthesis III (from α-maltose 1-phosphate)	8	3	1
valproate β-oxidation	9	7	1
benzoate biosynthesis I (CoA-dependent, β-oxidative)	9	4	1
phenylacetate degradation I (aerobic)	9	3	1
starch biosynthesis	10	5	1
3-phenylpropanoate degradation	10	5	1
superpathway of phenylethylamine degradation	11	5	1
Spodoptera littoralis pheromone biosynthesis	22	4	2
oleate β-oxidation	35	30	3
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase)	13	2	1
superpathway of glyoxylate cycle and fatty acid degradation	14	11	1
docosahexaenoate biosynthesis III (6-desaturase, mammals)	14	2	1
platensimycin biosynthesis	26	6	1