Experiment set6IT093 for Pseudomonas fluorescens FW300-N2C3

4C survival; 672 hrs

Group: survival
Media: LB
Culturing: pseudo5_N2-C3_1_ML2, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 6.7 generations
By: Jayashree on 10/28/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 16 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Alginate metabolism	2
Bacterial Chemotaxis	2
Flagellar motility	2
Terminal cytochrome d ubiquinol oxidases	2
Terminal cytochrome oxidases	2
Two-component regulatory systems in Campylobacter	2
Flagellum	1
Glycogen metabolism	1
Mannose Metabolism	1
Methylcitrate cycle	1
Murein hydrolase regulation and cell death	1
Propionate-CoA to Succinate Module	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Fructose and mannose metabolism
• Starch and sucrose metabolism
• Glycolysis / Gluconeogenesis
• Pentose phosphate pathway
• Galactose metabolism
• Streptomycin biosynthesis
• Propanoate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-α-D-glucose biosynthesis	2	2	1
glycogen biosynthesis I (from ADP-D-Glucose)	4	3	2
alginate biosynthesis I (algal)	3	3	1
aerobic respiration III (alternative oxidase pathway)	3	3	1
trehalose degradation V	3	2	1
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation	3	2	1
GDP-α-D-glucose biosynthesis	3	2	1
GDP-mannose biosynthesis	4	4	1
starch degradation V	4	3	1
sucrose degradation IV (sucrose phosphorylase)	4	3	1
starch degradation III	4	2	1
dTDP-β-L-rhamnose biosynthesis	5	5	1
2-methylcitrate cycle I	5	5	1
sucrose degradation II (sucrose synthase)	5	4	1
glucose and glucose-1-phosphate degradation	5	4	1
D-galactose degradation I (Leloir pathway)	5	3	1
starch biosynthesis	10	5	2
glucosylglycerol biosynthesis	5	2	1
CDP-6-deoxy-D-gulose biosynthesis	5	1	1
colanic acid building blocks biosynthesis	11	9	2
2-methylcitrate cycle II	6	5	1
glycogen degradation II	6	5	1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	5	1
alginate biosynthesis II (bacterial)	7	6	1
β-(1,4)-mannan degradation	7	2	1
sucrose biosynthesis II	8	6	1
glycogen degradation I	8	6	1
glycogen biosynthesis III (from α-maltose 1-phosphate)	8	3	1
sucrose biosynthesis I (from photosynthesis)	9	7	1
chitin biosynthesis	9	6	1
O-antigen building blocks biosynthesis (E. coli)	11	10	1
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis	14	7	1
streptomycin biosynthesis	18	2	1
superpathway of anaerobic sucrose degradation	19	15	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	5	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1