Experiment set6IT089 for Pseudomonas fluorescens FW300-N2E3
L-Arginine nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_Glucose_noNitrogen + L-Arginine (2.5 mM), pH=7
Culturing: pseudo3_N2E3_ML2, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 6.5 generations
By: Jayashree on 11/19/2014
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 1 genes in this experiment
For nitrogen source L-Arginine in Pseudomonas fluorescens FW300-N2E3
For nitrogen source L-Arginine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glycine and Serine Utilization | 1 |
| Pyridoxin (Vitamin B6) Biosynthesis | 1 |
| Serine Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-serine biosynthesis I | 3 | 3 | 1 |
| L-cysteine biosynthesis IX (Trichomonas vaginalis) | 3 | 2 | 1 |
| superpathway of L-serine and glycine biosynthesis I | 4 | 4 | 1 |
| pyridoxal 5'-phosphate biosynthesis I | 7 | 7 | 1 |
| L-lysine biosynthesis I | 9 | 9 | 1 |
| superpathway of sulfate assimilation and cysteine biosynthesis | 9 | 9 | 1 |
| superpathway of pyridoxal 5'-phosphate biosynthesis and salvage | 12 | 11 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis I | 18 | 16 | 1 |
| aspartate superpathway | 25 | 22 | 1 |