Experiment set6IT089 for Ralstonia sp. UNC404CL21Col

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Plant=Sorghum_bicolor; PlantTreatment=None; Sample=soil; GrowthSubstrate=calcine_clay; Collection=outgrowth_in_LB

Group: in planta
Media: MS_media (0.5x) + Plant=Sorghum_bicolor; PlantTreatment=None; Sample=soil; GrowthSubstrate=calcine_clay; Collection=outgrowth_in_LB
Culturing: CL21_ML4, pot, Aerobic, at 26 (C), (Solid)
By: Dawn on 16-Jun-19
Media components: 825 mg/L Ammonium Nitrate, 3.1 mg/L Boric Acid, 166.1 mg/L Calcium chloride, 0.0125 mg/L Cobalt chloride hexahydrate, 0.0125 mg/L Copper (II) sulfate pentahydrate, 18.63 mg/L EDTA (disodium salt), 13.9 mg/L Iron (II) sulfate heptahydrate, 1 mg/L Glycine, 90.35 mg/L Magnesium sulfate, 8.45 mg/L Manganese sulfate, 50 mg/L m-Inositol, 0.25 mg/L Nicotinic Acid, 0.415 mg/L Potassium iodide, 950 mg/L Potassium nitrate, 85 mg/L Potassium phosphate monobasic, 0.25 mg/L Pyridoxine HCl, 0.125 mg/L Sodium Molybdate Dihydrate, 0.05 mg/L Thiamine HCl, 4.3 mg/L Zinc sulfate heptahydrate, 10 g/L Sucrose (final concentrations)

Specific Phenotypes

For 1 genes in this experiment

For in planta Plant=Sorghum_bicolor; PlantTreatment=None; Sample=soil; GrowthSubstrate=calcine_clay; Collection=outgrowth_in_LB in Ralstonia sp. UNC404CL21Col

For in planta Plant=Sorghum_bicolor; PlantTreatment=None; Sample=soil; GrowthSubstrate=calcine_clay; Collection=outgrowth_in_LB across organisms

SEED Subsystems

Subsystem #Specific
Entner-Doudoroff Pathway 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Methylglyoxal Metabolism 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
ethylene glycol degradation 2 1 1
putrescine degradation V 2 1 1
putrescine degradation I 2 1 1
phenylethylamine degradation I 2 1 1
phenylethylamine degradation II 2 1 1
phenylethanol degradation 2 1 1
ethanol degradation IV 3 3 1
ethanol degradation II 3 3 1
L-phenylalanine degradation II (anaerobic) 3 2 1
hypotaurine degradation 3 2 1
ethanol degradation III 3 2 1
putrescine degradation IV 3 2 1
histamine degradation 3 1 1
styrene degradation 3 1 1
phytol degradation 4 3 1
fatty acid α-oxidation I (plants) 4 2 1
putrescine degradation III 4 2 1
L-tryptophan degradation X (mammalian, via tryptamine) 4 1 1
D-arabinose degradation II 4 1 1
mitochondrial NADPH production (yeast) 5 3 1
octane oxidation 5 3 1
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
dopamine degradation 5 1 1
3-methyl-branched fatty acid α-oxidation 6 3 1
alkane oxidation 6 1 1
noradrenaline and adrenaline degradation 13 4 2
superpathway of glycol metabolism and degradation 7 5 1
serotonin degradation 7 3 1
ceramide degradation by α-oxidation 7 2 1
limonene degradation IV (anaerobic) 7 1 1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 6 1
superpathway of ornithine degradation 8 3 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
aromatic biogenic amine degradation (bacteria) 8 1 1
Entner-Doudoroff pathway II (non-phosphorylative) 9 5 1
L-phenylalanine degradation IV (mammalian, via side chain) 9 4 1
superpathway of phenylethylamine degradation 11 8 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 5 1
superpathway of L-arginine and L-ornithine degradation 13 7 1
anaerobic aromatic compound degradation (Thauera aromatica) 27 5 1
superpathway of pentose and pentitol degradation 42 17 1