Experiment set6IT088 for Pseudomonas fluorescens FW300-N2E2
LB with Magnesium chloride hexahydrate 0.125 M
Group: stressMedia: LB + Magnesium chloride hexahydrate (0.125 M), pH=7
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Hans_Hualan on 2/2/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Specific Phenotypes
For 12 genes in this experiment
For stress Magnesium chloride hexahydrate in Pseudomonas fluorescens FW300-N2E2
For stress Magnesium chloride hexahydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| DNA repair, UvrABC system | 1 |
| Peptidoglycan Biosynthesis | 1 |
| Polyamine Metabolism | 1 |
| Transport of Iron | 1 |
| Type IV pilus | 1 |
| cAMP signaling in bacteria | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycine, serine and threonine metabolism
- Lysine degradation
- Nucleotide sugars metabolism
- Peptidoglycan biosynthesis
- Fructose and mannose metabolism
- Ascorbate and aldarate metabolism
- C21-Steroid hormone metabolism
- Methionine metabolism
- Lysine biosynthesis
- Arginine and proline metabolism
- Bisphenol A degradation
- Aminophosphonate metabolism
- Polyketide sugar unit biosynthesis
- Aminosugars metabolism
- Linoleic acid metabolism
- Tetrachloroethene degradation
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Biotin metabolism
- Retinol metabolism
- Caprolactam degradation
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of type II polyketide products
- Biosynthesis of alkaloids derived from shikimate pathway
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| ectoine degradation | 4 | 1 | 1 |
| ectoine biosynthesis | 5 | 3 | 1 |
| norspermidine biosynthesis | 6 | 4 | 1 |
| rhizobactin 1021 biosynthesis | 7 | 1 | 1 |
| superpathway of polyamine biosynthesis III | 8 | 4 | 1 |
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 12 | 2 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 12 | 2 |
| peptidoglycan biosynthesis V (β-lactam resistance) | 17 | 11 | 2 |
| pyoverdine I biosynthesis | 11 | 7 | 1 |
| baumannoferrin biosynthesis | 11 | 1 | 1 |
| peptidoglycan biosynthesis I (meso-diaminopimelate containing) | 12 | 12 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 4 | 1 |
| peptidoglycan biosynthesis III (mycobacteria) | 15 | 11 | 1 |