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Experiment set6IT080 for
Pseudomonas fluorescens FW300-N2C3
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Minimal media pH7
200 most important genes:
gene
name
fitness
t score
description
AO356
_14435
-5.7
-7.8
anthranilate phosphoribosyltransferase
compare
AO356
_21405
-5.7
-5.5
3-isopropylmalate dehydratase
compare
AO356
_09815
-5.7
-6.8
imidazoleglycerol-phosphate dehydratase
compare
AO356
_16175
-5.5
-6.5
acetolactate synthase 3 regulatory subunit
compare
AO356
_14440
-5.3
-6.3
indole-3-glycerol-phosphate synthase
compare
AO356
_09520
-5.3
-5.2
phosphoribosyl-AMP cyclohydrolase
compare
AO356
_09525
-5.3
-7.2
phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)
(from data)
compare
AO356
_21440
-5.1
-10.9
N-(5'-phosphoribosyl)anthranilate isomerase
compare
AO356
_12520
-5.1
-3.5
orotate phosphoribosyltransferase
compare
AO356
_14420
-5.0
-16.4
anthranilate synthase
compare
AO356
_13605
-4.9
-8.2
5,10-methylenetetrahydrofolate reductase
compare
AO356
_08635
-4.9
-11.4
ATP phosphoribosyltransferase regulatory subunit
compare
AO356
_21465
-4.9
-5.8
amidophosphoribosyltransferase
compare
AO356
_19090
-4.9
-3.3
phosphoribosylaminoimidazole synthetase
compare
AO356
_13250
-4.8
-11.7
dihydroxy-acid dehydratase
compare
AO356
_22200
-4.8
-9.8
sirohydrochlorin ferrochelatase
compare
AO356
_13410
-4.8
-13.8
homoserine O-acetyltransferase
compare
AO356
_00555
-4.7
-12.1
sulfite reductase
compare
AO356
_15165
-4.7
-4.5
protein cII
compare
AO356
_13405
-4.7
-11.1
methionine biosynthesis protein MetW
compare
AO356
_18325
-4.7
-15.2
argininosuccinate synthase
compare
AO356
_19795
-4.6
-3.2
glycerol-3-phosphate dehydrogenase
compare
AO356
_21400
-4.6
-6.0
isopropylmalate isomerase
compare
AO356
_12525
-4.5
-11.7
acetylglutamate kinase
compare
AO356
_10910
-4.5
-10.5
tryptophan synthase subunit alpha
compare
AO356
_16170
-4.5
-13.3
acetolactate synthase 3 catalytic subunit
compare
AO356
_12020
-4.4
-15.4
pyruvate carboxylase subunit A
compare
AO356
_16180
-4.4
-16.3
ketol-acid reductoisomerase
compare
AO356
_08735
-4.4
-15.4
phosphoserine phosphatase
compare
AO356
_18275
-4.4
-15.0
ornithine carbamoyltransferase
compare
AO356
_14295
-4.4
-3.0
2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase
compare
AO356
_06970
-4.3
-8.1
ATP phosphoribosyltransferase (EC 2.4.2.17)
(from data)
compare
AO356
_09370
-4.3
-11.8
glutamate synthase
compare
AO356
_13085
-4.3
-12.4
N-acetylglutamate synthase
compare
AO356
_09375
-4.3
-21.2
glutamate synthase
compare
AO356
_10915
-4.3
-11.7
tryptophan synthase subunit beta
compare
AO356
_21415
-4.2
-11.8
3-isopropylmalate dehydrogenase
compare
AO356
_05160
-4.2
-9.3
glucose-6-phosphate dehydrogenase
compare
AO356
_06965
-4.1
-10.6
histidinol dehydrogenase
compare
AO356
_10340
-4.1
-15.0
phosphoserine phosphatase
compare
AO356
_09810
-4.1
-8.2
imidazole glycerol phosphate synthase subunit HisH
compare
AO356
_12015
-4.0
-13.2
LysR family transcriptional regulator
compare
AO356
_18730
-4.0
-12.3
arginine N-succinyltransferase
compare
AO356
_16075
-4.0
-5.4
Fis family transcriptional regulator
compare
AO356
_12025
-3.8
-14.6
pyruvate carboxylase subunit B
compare
AO356
_21470
-3.7
-7.4
O-succinylhomoserine sulfhydrylase
compare
AO356
_11315
-3.7
-2.6
shikimate dehydrogenase
compare
AO356
_09800
-3.7
-6.7
1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16)
(from data)
compare
AO356
_07480
-3.7
-4.4
carbamoyl phosphate synthase large subunit
compare
AO356
_14430
-3.7
-7.6
anthranilate synthase
compare
AO356
_16870
-3.6
-11.3
benzoate transporter
compare
AO356
_16050
-3.6
-3.5
glucose-6-phosphate isomerase
compare
AO356
_22795
-3.5
-5.3
UTP--glucose-1-phosphate uridylyltransferase
compare
AO356
_02175
-3.5
-8.6
transaldolase
compare
AO356
_06550
-3.4
-8.9
2-isopropylmalate synthase
compare
AO356
_12780
-3.3
-8.4
argininosuccinate lyase
compare
AO356
_14135
-3.3
-15.5
biotin synthase
compare
AO356
_09795
-3.3
-2.2
imidazole glycerol phosphate synthase subunit HisF
compare
AO356
_10300
-3.3
-4.2
3-phosphoglycerate dehydrogenase
compare
AO356
_08905
-3.2
-4.3
glycosyl transferase
compare
AO356
_05225
-3.2
-7.4
glyceraldehyde-3-phosphate dehydrogenase
compare
AO356
_00560
-3.1
-3.7
required for sulfate utilization, putative electron transport protein for sulfite reductase
(from data)
compare
AO356
_16955
-3.1
-9.2
disulfide bond formation protein B
compare
AO356
_05215
-3.1
-7.9
glucokinase
compare
AO356
_17890
-3.0
-4.1
H-NS histone
compare
AO356
_05055
-2.9
-7.4
permease DsdX
compare
AO356
_20830
-2.8
-5.0
phosphoadenosine phosphosulfate reductase
compare
AO356
_17520
-2.8
-2.7
phosphoribosylformylglycinamidine synthase
compare
AO356
_27180
-2.8
-5.8
hypothetical protein
compare
AO356
_27215
-2.8
-5.1
hypothetical protein
compare
AO356
_17830
-2.7
-6.2
pyruvate kinase
compare
AO356
_05060
-2.7
-3.7
gluconokinase
compare
AO356
_21330
-2.7
-11.1
aromatic amino acid aminotransferase
compare
AO356
_27210
-2.7
-9.4
hypothetical protein
compare
AO356
_06960
-2.5
-7.6
histidinol-phosphate aminotransferase
compare
AO356
_10920
-2.5
-8.3
LysR family transcriptional regulator
compare
AO356
_17950
-2.5
-10.2
histidine kinase
compare
AO356
_17075
-2.5
-3.7
cytochrome C
compare
AO356
_01740
-2.4
-4.8
DNA-binding protein
compare
AO356
_18025
-2.4
-3.6
ATP-dependent DNA helicase RuvB
compare
AO356
_15815
-2.4
-2.1
gamma-glutamyl kinase
compare
AO356
_24845
-2.2
-3.8
ATPase
compare
AO356
_12900
-2.1
-5.3
exopolyphosphatase
compare
AO356
_19520
-2.1
-3.9
isochorismatase
compare
AO356
_14140
-2.0
-10.5
8-amino-7-oxononanoate synthase
compare
AO356
_14150
-2.0
-8.8
malonyl-[acyl-carrier protein] O-methyltransferase BioC
compare
AO356
_13570
-2.0
-9.3
adenosylmethionine-8-amino-7-oxononanoate aminotransferase
compare
AO356
_00355
-2.0
-7.2
chemotaxis protein CheY
compare
AO356
_18775
-1.9
-2.8
aspartate kinase
compare
AO356
_19045
-1.9
-8.6
chemotaxis protein CheY
compare
AO356
_08585
-1.9
-4.1
50S ribosomal protein L9
compare
AO356
_01745
-1.8
-5.6
ATP-dependent Clp protease ATP-binding subunit ClpX
compare
AO356
_14145
-1.8
-5.1
transporter
compare
AO356
_06575
-1.8
-3.4
outer membrane protein assembly factor BamB
compare
AO356
_14155
-1.8
-7.1
dethiobiotin synthetase
compare
AO356
_27205
-1.8
-6.6
hypothetical protein
compare
AO356
_08895
-1.8
-2.5
GlcNAc-PI de-N-acetylase
compare
AO356
_08465
-1.7
-3.3
protein hupE
compare
AO356
_18735
-1.7
-4.9
arginine N-succinyltransferase
compare
AO356
_20175
-1.6
-2.7
3-oxoacyl-ACP synthase
compare
AO356
_27230
-1.6
-4.0
hypothetical protein
compare
AO356
_16850
-1.5
-8.7
serine hydroxymethyltransferase
compare
AO356
_15420
-1.5
-4.4
hypothetical protein
compare
AO356
_07370
-1.5
-5.2
nicotinate-nucleotide pyrophosphorylase
compare
AO356
_05195
-1.5
-8.5
sugar ABC transporter substrate-binding protein
compare
AO356
_05180
-1.5
-5.1
sugar ABC transporter ATP-binding protein
compare
AO356
_05185
-1.5
-6.9
sugar ABC transporter permease
compare
AO356
_12005
-1.5
-8.7
transcriptional regulator
compare
AO356
_18000
-1.5
-1.6
FmdB family transcriptional regulator
compare
AO356
_19420
-1.5
-2.0
septum formation inhibitor
compare
AO356
_19085
-1.4
-7.2
phosphoribosylglycinamide formyltransferase
compare
AO356
_05190
-1.4
-10.2
sugar ABC transporter permease
compare
AO356
_13700
-1.4
-5.1
hypothetical protein
compare
AO356
_18020
-1.4
-3.4
ATP-dependent DNA helicase RuvA
compare
AO356
_04560
-1.4
-3.1
serine peptidase
compare
AO356
_00500
-1.4
-4.4
magnesium chelatase
compare
AO356
_16070
-1.4
-5.8
poly(A) polymerase
compare
AO356
_12735
-1.3
-2.3
diaminopimelate epimerase
compare
AO356
_28300
-1.3
-1.6
hypothetical protein
compare
AO356
_10005
-1.3
-7.2
branched-chain amino acid permease (azaleucine resistance)
compare
AO356
_20475
-1.3
-2.3
hypothetical protein
compare
AO356
_07540
-1.3
-2.2
SsrA-binding protein
compare
AO356
_26210
-1.3
-1.9
histidine kinase
compare
AO356
_13445
-1.3
-1.4
dihydroorotase
compare
AO356
_05205
-1.3
-5.6
histidine kinase
compare
AO356
_06995
-1.3
-4.1
outer membrane lipid asymmetry maintenance protein MlaD
compare
AO356
_08280
-1.3
-3.9
oxidoreductase
compare
AO356
_03435
-1.3
-2.9
integration host factor subunit beta
compare
AO356
_19135
-1.3
-3.0
cob(I)yrinic acid a,c-diamide adenosyltransferase
compare
AO356
_06385
-1.3
-3.3
recombinase XerD
compare
AO356
_27250
-1.3
-3.5
hypothetical protein
compare
AO356
_02315
-1.3
-3.7
DNA topoisomerase I
compare
AO356
_05210
-1.3
-3.7
chemotaxis protein CheY
compare
AO356
_22405
-1.3
-2.5
hypothetical protein
compare
AO356
_02305
-1.2
-1.5
hypothetical protein
compare
AO356
_10330
-1.2
-8.8
threonine dehydratase
compare
AO356
_06005
-1.2
-4.0
zinc metallopeptidase RseP
compare
AO356
_28695
-1.2
-2.3
IclR family transcriptional regulator
compare
AO356
_03180
-1.2
-3.3
CysB family transcriptional regulator
compare
AO356
_21410
-1.2
-1.4
SAM-dependent methyltransferase
compare
AO356
_17070
-1.1
-2.2
cytochrome B
compare
AO356
_01750
-1.1
-3.2
ATP-dependent Clp protease proteolytic subunit
compare
AO356
_08235
-1.1
-5.4
histidine kinase
compare
AO356
_12380
-1.1
-5.9
ATP-dependent DNA helicase RecG
compare
AO356
_19140
-1.1
-7.2
cobyrinic acid a,c-diamide synthase
compare
AO356
_18910
-1.1
-6.6
chemotaxis protein
compare
AO356
_02290
-1.1
-2.3
TetR family transcriptional regulator
compare
AO356
_11990
-1.1
-7.3
DNA helicase II
compare
AO356
_25390
-1.1
-2.3
enamine deaminase RidA
compare
AO356
_08890
-1.1
-5.0
glycosyltransferase
compare
AO356
_16670
-1.1
-6.6
adenylylsulfate kinase
compare
AO356
_16665
-1.1
-7.0
sulfate adenylyltransferase
compare
AO356
_17065
-1.1
-1.9
ubiquinol-cytochrome c reductase iron-sulfur subunit
compare
AO356
_03725
-1.1
-2.3
histidine kinase
compare
AO356
_21795
-1.1
-2.3
PBS lyase
compare
AO356
_07920
-1.0
-3.8
exodeoxyribonuclease V subunit gamma
compare
AO356
_20335
-1.0
-2.9
hypothetical protein
compare
AO356
_10075
-1.0
-3.6
3'(2'),5'-bisphosphate nucleotidase CysQ
compare
AO356
_02800
-1.0
-2.9
transporter
compare
AO356
_14540
-1.0
-1.6
biotin--protein ligase
compare
AO356
_08290
-1.0
-3.2
precorrin-6Y C5,15-methyltransferase
compare
AO356
_30345
-1.0
-3.8
IclR family transcriptional regulator
compare
AO356
_12990
-1.0
-4.1
5-formyltetrahydrofolate cyclo-ligase
compare
AO356
_18370
-1.0
-3.4
NADH:quinone oxidoreductase
compare
AO356
_07910
-1.0
-1.6
exodeoxyribonuclease V subunit alpha
compare
AO356
_06690
-1.0
-2.7
queuine tRNA-ribosyltransferase
compare
AO356
_07915
-1.0
-2.6
exodeoxyribonuclease V subunit beta
compare
AO356
_08670
-1.0
-1.9
N-acetylmuramoyl-L-alanine amidase
compare
AO356
_08610
-1.0
-4.8
exoribonuclease R
compare
AO356
_19165
-1.0
-2.9
adenosylcobinamide kinase
compare
AO356
_12725
-1.0
-2.7
recombinase XerC
compare
AO356
_17825
-1.0
-4.2
enoyl-CoA hydratase
compare
AO356
_28685
-1.0
-2.5
betaine-aldehyde dehydrogenase
compare
AO356
_27400
-1.0
-2.7
1,2-oxophytodienoate reductase
compare
AO356
_10000
-0.9
-3.5
hypothetical protein
compare
AO356
_27245
-0.9
-0.7
hypothetical protein
compare
AO356
_19050
-0.9
-2.1
cysteine synthase
compare
AO356
_13695
-0.9
-2.3
hypothetical protein
compare
AO356
_16080
-0.9
-4.8
ATPase
compare
AO356
_03800
-0.9
-1.7
histidine kinase
compare
AO356
_11450
-0.9
-1.3
cytochrome C
compare
AO356
_08300
-0.9
-3.0
cobalt-precorrin-6X reductase
compare
AO356
_00530
-0.9
-2.9
precorrin-4 C11-methyltransferase
compare
AO356
_10585
-0.9
-6.1
hypothetical protein
compare
AO356
_26475
-0.9
-2.1
lactoylglutathione lyase
compare
AO356
_19155
-0.9
-5.3
threonine-phosphate decarboxylase
compare
AO356
_06990
-0.8
-3.3
toluene tolerance protein
compare
AO356
_08275
-0.8
-2.1
precorrin isomerase
compare
AO356
_11465
-0.8
-5.2
DNA polymerase I
compare
AO356
_25440
-0.8
-3.5
histidine kinase
compare
AO356
_13685
-0.8
-1.4
glyceraldehyde-3-phosphate dehydrogenase
compare
AO356
_18480
-0.8
-1.0
translation initiation factor 2
compare
AO356
_27240
-0.8
-2.1
hypothetical protein
compare
AO356
_23325
-0.8
-2.9
short-chain dehydrogenase
compare
AO356
_07005
-0.8
-1.4
ABC transporter ATP-binding protein
compare
AO356
_18725
-0.8
-2.6
acetylornithine aminotransferase
compare
AO356
_18145
-0.8
-4.3
quinolinate synthase A
compare
AO356
_03070
-0.8
-5.5
histidine kinase
compare
AO356
_28160
-0.8
-1.9
hypothetical protein
compare
AO356
_02190
-0.8
-3.9
hypothetical protein
compare
Specific Phenotypes
None in this experiment
For Pseudomonas fluorescens FW300-N2C3 in pH experiments