Experiment set6IT075 for Pseudomonas fluorescens FW300-N2E2

LB with Sodium thiosulfate pentahydrate 0.25 M

Group: stress
Media: LB + Sodium thiosulfate pentahydrate (0.25 M), pH=7
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Hans_Hualan on 2/2/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 9 genes in this experiment

For stress Sodium thiosulfate pentahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Sodium thiosulfate pentahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Peptidoglycan Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Puromycin biosynthesis
• Glycosaminoglycan degradation
• Peptidoglycan biosynthesis
• alpha-Linolenic acid metabolism
• Trinitrotoluene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
queuosine biosynthesis I (de novo)	4	4	1
queuosine biosynthesis III (queuosine salvage)	5	3	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	2
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	1
peptidoglycan biosynthesis II (staphylococci)	17	12	1