Experiment set6IT072 for Bifidobacterium breve UCC2003

reconstituted MRS with L-Fucose 30 mM

Group: carbon source
Media: reconstituted MRS + L-Fucose (30 mM), pH=6.8
Culturing: Bifido_ML2, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Anthony Shiver on 7/16/2020

Specific Phenotypes

For 7 genes in this experiment

For carbon source L-Fucose in Bifidobacterium breve UCC2003

For carbon source L-Fucose across organisms

SEED Subsystems

Subsystem	#Specific
L-fucose utilization temp	2

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid biosynthesis
• Lysine biosynthesis
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
gondoate biosynthesis (anaerobic)	4	3	1
dipicolinate biosynthesis	4	3	1
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast)	12	6	3
D-xylose degradation to ethylene glycol (engineered)	4	1	1
palmitate biosynthesis III	29	14	7
tetradecanoate biosynthesis (mitochondria)	25	11	6
palmitate biosynthesis II (type II fatty acid synthase)	31	22	7
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate)	9	6	2
oleate biosynthesis IV (anaerobic)	14	9	3
superpathway of fatty acids biosynthesis (E. coli)	53	35	11
fatty acid elongation -- saturated	5	3	1
superpathway of unsaturated fatty acids biosynthesis (E. coli)	20	11	4
cis-vaccenate biosynthesis	5	2	1
8-amino-7-oxononanoate biosynthesis IV	5	2	1
L-fucose degradation II	5	2	1
superpathway of fatty acid biosynthesis II (plant)	43	29	8
8-amino-7-oxononanoate biosynthesis I	11	6	2
anteiso-branched-chain fatty acid biosynthesis	34	13	6
even iso-branched-chain fatty acid biosynthesis	34	13	6
odd iso-branched-chain fatty acid biosynthesis	34	13	6
stearate biosynthesis II (bacteria and plants)	6	4	1
(5Z)-dodecenoate biosynthesis I	6	3	1
stearate biosynthesis IV	6	3	1
(5Z)-dodecenoate biosynthesis II	6	3	1
petroselinate biosynthesis	6	1	1
streptorubin B biosynthesis	34	14	5
L-lysine biosynthesis VI	7	6	1
L-lysine biosynthesis III	7	6	1
D-xylose degradation IV	7	1	1
biotin biosynthesis I	15	6	2
superpathway of fatty acid biosynthesis I (E. coli)	16	10	2
L-fucose degradation III	8	3	1
2-allylmalonyl-CoA biosynthesis	8	1	1
L-lysine biosynthesis I	9	9	1
Entner-Doudoroff pathway II (non-phosphorylative)	9	6	1
L-lysine biosynthesis II	9	6	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	16	1
aspartate superpathway	25	23	1
mycolate biosynthesis	205	17	5
superpathway of pentose and pentitol degradation	42	4	1
superpathway of mycolate biosynthesis	239	18	5