Experiment set6IT071 for Klebsiella michiganensis M5al

Thymine nitrogen source

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + Thymine (2 mM), pH=7
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Apr-17
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1811 C4

Specific Phenotypes

For 11 genes in this experiment

For nitrogen source Thymine in Klebsiella michiganensis M5al

For nitrogen source Thymine across organisms

SEED Subsystems

Subsystem	#Specific
Molybdenum cofactor biosynthesis	2
Nitrate and nitrite ammonification	2
DNA-replication	1
Nitrosative stress	1
Oxidative stress	1
Phosphate metabolism	1
Pyrimidine utilization	1
Rrf2 family transcriptional regulators	1
Stringent Response, (p)ppGpp metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lipopolysaccharide biosynthesis
• Purine metabolism
• Puromycin biosynthesis
• Nucleotide sugars metabolism
• Biosynthesis of siderophore group nonribosomal peptides

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
bis(guanylyl molybdenum cofactor) biosynthesis	2	2	2
bis(guanylyl tungstenpterin) cofactor biosynthesis	1	1	1
guanylyl molybdenum cofactor biosynthesis	1	1	1
molybdenum cofactor biosynthesis	3	3	1
bis(tungstenpterin) cofactor biosynthesis	4	1	1
ppGpp metabolism	6	6	1
molybdopterin biosynthesis	6	5	1