Experiment set6IT067 for Pseudomonas fluorescens FW300-N2E2

LB with Sodium deoxycholate monohydrate 0.0125 M

Group: stress
Media: LB + Sodium deoxycholate monohydrate (0.0125 M), pH=7
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Hans_Hualan on 2/2/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 11 genes in this experiment

For stress Sodium deoxycholate monohydrate in Pseudomonas fluorescens FW300-N2E2

For stress Sodium deoxycholate monohydrate across organisms

SEED Subsystems

Subsystem	#Specific
Lipid A modifications	1
Lysine degradation	1
Peptidoglycan Biosynthesis	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Peptidoglycan biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	2
peptidoglycan biosynthesis II (staphylococci)	17	12	1
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	1