Experiment set6IT066 for Klebsiella michiganensis M5al
Adenosine nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_Glucose_noNitrogen + Adenosine (2 mM), pH=7
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Apr-17
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1811 B4
Specific Phenotypes
For 4 genes in this experiment
For nitrogen source Adenosine in Klebsiella michiganensis M5al
For nitrogen source Adenosine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Hydantoin metabolism | 1 |
| Photorespiration (oxidative C2 cycle) | 1 |
| Pyruvate Alanine Serine Interconversions | 1 |
| Serine-glyoxylate cycle | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycine, serine and threonine metabolism
- Ubiquinone and menaquinone biosynthesis
- Purine metabolism
- Alanine and aspartate metabolism
- Methionine metabolism
- Lysine biosynthesis
- Lysine degradation
- Arginine and proline metabolism
- Histidine metabolism
- Phenylalanine metabolism
- gamma-Hexachlorocyclohexane degradation
- Bisphenol A degradation
- Tryptophan metabolism
- Aminophosphonate metabolism
- Cyanoamino acid metabolism
- Nucleotide sugars metabolism
- Toluene and xylene degradation
- 2,4-Dichlorobenzoate degradation
- 1- and 2-Methylnaphthalene degradation
- Naphthalene and anthracene degradation
- 1,4-Dichlorobenzene degradation
- Benzoate degradation via CoA ligation
- Styrene degradation
- Methane metabolism
- Limonene and pinene degradation
- Brassinosteroid biosynthesis
- Carotenoid biosynthesis - General
- Caprolactam degradation
- Phenylpropanoid biosynthesis
- Flavonoid biosynthesis
- Isoflavonoid biosynthesis
- Alkaloid biosynthesis I
- Biosynthesis of phenylpropanoids
- Biosynthesis of terpenoids and steroids
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycine biosynthesis III | 1 | 1 | 1 |
| vanillin and vanillate degradation II | 2 | 2 | 1 |
| allantoin degradation to ureidoglycolate II (ammonia producing) | 4 | 4 | 1 |
| L-serine biosynthesis II | 4 | 3 | 1 |
| allantoin degradation to glyoxylate III | 5 | 5 | 1 |
| allantoin degradation to glyoxylate II | 5 | 5 | 1 |
| superpathway of allantoin degradation in plants | 8 | 7 | 1 |
| allantoin degradation IV (anaerobic) | 9 | 8 | 1 |