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Experiment set6IT061 for
Pseudomonas fluorescens FW300-N2E3
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Minimal media pH8
200 most important genes:
gene
name
fitness
t score
description
AO353
_10710
-4.5
-4.4
tryptophan synthase subunit beta
compare
AO353
_02070
-4.5
-7.5
prephenate dehydratase
compare
AO353
_20635
-4.3
-14.6
3-isopropylmalate dehydrogenase
compare
AO353
_14505
-4.3
-12.7
ketol-acid reductoisomerase
compare
AO353
_13070
-4.3
-10.3
phosphoserine phosphatase
compare
AO353
_08185
-4.3
-11.7
methionine biosynthesis protein MetW
compare
AO353
_05115
-4.2
-13.2
ATP phosphoribosyltransferase (EC 2.4.2.17)
(from data)
compare
AO353
_20625
-4.2
-5.7
3-isopropylmalate dehydratase
compare
AO353
_12365
-4.2
-5.7
phosphoribosyl-AMP cyclohydrolase
compare
AO353
_08885
-4.1
-21.2
polyphosphate kinase
compare
AO353
_12520
-4.1
-13.8
glutamate synthase
compare
AO353
_12515
-4.1
-24.6
glutamate synthase
compare
AO353
_20695
-4.0
-8.9
O-succinylhomoserine sulfhydrylase
compare
AO353
_12085
-4.0
-9.7
1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16)
(from data)
compare
AO353
_20665
-4.0
-12.6
N-(5'-phosphoribosyl)anthranilate isomerase
compare
AO353
_08475
-4.0
-11.1
phosphoserine phosphatase
compare
AO353
_07210
-3.9
-7.4
indole-3-glycerol-phosphate synthase
compare
AO353
_07230
-3.9
-19.7
anthranilate synthase
compare
AO353
_12070
-3.9
-11.4
imidazoleglycerol-phosphate dehydratase
compare
AO353
_02075
-3.9
-14.0
3-phosphoserine/phosphohydroxythreonine aminotransferase
compare
AO353
_20540
-3.8
-10.3
aromatic amino acid aminotransferase
compare
AO353
_12360
-3.8
-10.9
phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)
(from data)
compare
AO353
_12090
-3.8
-3.7
imidazole glycerol phosphate synthase subunit HisF
compare
AO353
_05110
-3.7
-14.2
histidinol dehydrogenase
compare
AO353
_08345
-3.6
-10.9
dihydroxy-acid dehydratase
compare
AO353
_08685
-3.6
-11.3
N-acetylglutamate synthase
compare
AO353
_00310
-3.6
-12.5
transaldolase
compare
AO353
_19060
-3.6
-7.5
CysB family transcriptional regulator
compare
AO353
_13165
-3.6
-7.1
ATP phosphoribosyltransferase regulatory subunit
compare
AO353
_09240
-3.5
-3.4
acetylglutamate kinase
compare
AO353
_08180
-3.4
-16.0
homoserine O-acetyltransferase
compare
AO353
_07220
-3.4
-10.9
anthranilate synthase
compare
AO353
_04105
-3.4
-11.6
argininosuccinate synthase
compare
AO353
_02710
-3.4
-16.7
(p)ppGpp synthetase
compare
AO353
_03360
-3.4
-11.8
glucose-6-phosphate dehydrogenase
compare
AO353
_20620
-3.4
-8.0
isopropylmalate isomerase
compare
AO353
_12075
-3.4
-10.4
imidazole glycerol phosphate synthase subunit HisH
compare
AO353
_07215
-3.3
-10.0
anthranilate phosphoribosyltransferase
compare
AO353
_10670
-3.3
-9.3
shikimate dehydrogenase
compare
AO353
_07155
-3.3
-10.7
N-acetyl-gamma-glutamyl-phosphate reductase
compare
AO353
_14495
-3.3
-14.6
acetolactate synthase 3 catalytic subunit
compare
AO353
_08165
-3.3
-4.5
pyrroline-5-carboxylate reductase
compare
AO353
_14775
-3.2
-10.7
hypothetical protein
compare
AO353
_12500
-3.2
-2.2
shikimate kinase
compare
AO353
_02685
-3.2
-6.2
phosphoribosylglycinamide formyltransferase
compare
AO353
_12105
-3.2
-10.5
peptidase S41
compare
AO353
_13100
-3.1
-3.7
GTPase RsgA
compare
AO353
_00825
-3.1
-7.0
porin
compare
AO353
_07515
-3.1
-3.6
transporter
compare
AO353
_22800
-3.0
-4.4
sulfite reductase
compare
AO353
_02250
-2.9
-5.7
chorismate synthase
compare
AO353
_14420
-2.9
-7.3
molecular chaperone DnaK
compare
AO353
_10030
-2.9
-13.4
pyruvate carboxylase subunit A
compare
AO353
_07520
-2.9
-16.0
8-amino-7-oxononanoate synthase
compare
AO353
_14400
-2.9
-6.0
Fis family transcriptional regulator
compare
AO353
_09000
-2.8
-8.3
argininosuccinate lyase
compare
AO353
_07525
-2.8
-14.3
biotin synthase
compare
AO353
_04155
-2.8
-12.8
ornithine carbamoyltransferase
compare
AO353
_08510
-2.8
-6.8
3-phosphoglycerate dehydrogenase
compare
AO353
_16940
-2.7
-8.2
deoxycytidine triphosphate deaminase
compare
AO353
_10025
-2.7
-16.5
pyruvate carboxylase subunit B
compare
AO353
_03415
-2.7
-8.3
glucokinase
compare
AO353
_07505
-2.7
-10.9
dethiobiotin synthetase
compare
AO353
_07510
-2.7
-12.6
malonyl-[acyl-carrier protein] O-methyltransferase BioC
compare
AO353
_08045
-2.7
-16.0
adenosylmethionine-8-amino-7-oxononanoate aminotransferase
compare
AO353
_09285
-2.7
-11.4
reactive intermediate/imine deaminase
compare
AO353
_14115
-2.7
-8.3
gamma-glutamyl kinase
compare
AO353
_08015
-2.6
-8.4
5,10-methylenetetrahydrofolate reductase
compare
AO353
_10035
-2.6
-9.1
LysR family transcriptional regulator
compare
AO353
_14595
-2.6
-11.3
carbonate dehydratase
compare
AO353
_05080
-2.6
-13.2
sulfate adenylyltransferase
compare
AO353
_08780
-2.6
-7.9
5-formyltetrahydrofolate cyclo-ligase
compare
AO353
_04355
-2.6
-3.6
homoserine dehydrogenase
compare
AO353
_14370
-2.5
-8.4
glucose-6-phosphate isomerase
compare
AO353
_15925
-2.5
-9.9
2-isopropylmalate synthase
compare
AO353
_14500
-2.5
-7.4
acetolactate synthase 3 regulatory subunit
compare
AO353
_05105
-2.5
-12.7
histidinol-phosphate aminotransferase
compare
AO353
_02975
-2.5
-4.9
aspartate kinase
compare
AO353
_05075
-2.5
-11.2
adenylylsulfate kinase
compare
AO353
_03525
-2.3
-10.1
quinolinate synthase A
compare
AO353
_06495
-2.3
-5.3
gamma-glutamyl phosphate reductase
compare
AO353
_05520
-2.2
-8.1
nicotinate-nucleotide pyrophosphorylase
compare
AO353
_11730
-2.2
-11.9
glutamate--cysteine ligase
compare
AO353
_20175
-2.1
-4.9
ATP-dependent Clp protease proteolytic subunit
compare
AO353
_12115
-2.1
-3.4
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
compare
AO353
_03020
-2.0
-7.3
arginine N-succinyltransferase
compare
AO353
_06455
-2.0
-3.9
D-alanyl-D-alanine carboxypeptidase
compare
AO353
_10705
-2.0
-4.2
tryptophan synthase subunit alpha
compare
AO353
_13640
-1.9
-4.8
histidine kinase
compare
AO353
_20170
-1.9
-5.0
trigger factor
compare
AO353
_03585
-1.9
-2.3
ATP-dependent DNA helicase RuvA
compare
AO353
_07355
-1.9
-7.8
2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase
compare
AO353
_10730
-1.8
-1.6
hypothetical protein
compare
AO353
_03615
-1.8
-4.1
cold-shock protein
compare
AO353
_03580
-1.8
-4.6
ATP-dependent DNA helicase RuvB
compare
AO353
_27650
-1.8
-5.2
cell division protein FtsK
compare
AO353
_05240
-1.7
-2.8
rod shape-determining protein MreD
compare
AO353
_19360
-1.7
-3.3
peroxiredoxin
compare
AO353
_11850
-1.7
-5.4
3'(2'),5'-bisphosphate nucleotidase CysQ
compare
AO353
_03775
-1.7
-8.5
pyruvate kinase
compare
AO353
_24130
-1.7
-4.3
hypothetical protein
compare
AO353
_23245
-1.7
-3.2
DNA-3-methyladenine glycosylase
compare
AO353
_08350
-1.6
-7.4
hypothetical protein
compare
AO353
_03420
-1.6
-4.2
phosphogluconate dehydratase
compare
AO353
_02720
-1.6
-5.8
cysteine synthase
compare
AO353
_05150
-1.6
-5.8
ABC transporter ATP-binding protein
compare
AO353
_14220
-1.6
-1.8
ribosomal large subunit pseudouridine synthase D
compare
AO353
_06045
-1.6
-7.1
exodeoxyribonuclease V subunit beta
compare
AO353
_06585
-1.6
-6.7
hypothetical protein
compare
AO353
_17035
-1.6
-6.2
hypothetical protein
compare
AO353
_00750
-1.6
-5.1
GntR family transcriptional regulator
compare
AO353
_08115
-1.5
-6.1
energy transducer TonB
compare
AO353
_12145
-1.5
-3.8
nitrogen regulation protein NR(I)
compare
AO353
_04520
-1.5
-4.9
elongation factor 4
compare
AO353
_04565
-1.4
-5.2
nucleoid-associated protein
compare
AO353
_05690
-1.4
-4.0
SsrA-binding protein
compare
AO353
_03590
-1.4
-4.0
crossover junction endodeoxyribonuclease RuvC
compare
AO353
_06050
-1.4
-6.1
exodeoxyribonuclease V subunit gamma
compare
AO353
_01375
-1.4
-6.1
phosphate acyltransferase
compare
AO353
_00495
-1.4
-1.9
DNA topoisomerase I
compare
AO353
_17620
-1.4
-10.1
L-aspartate oxidase
compare
AO353
_01780
-1.4
-8.0
heat-shock protein Hsp90
compare
AO353
_14740
-1.3
-3.4
GTP-binding protein
compare
AO353
_11770
-1.3
-2.2
molecular chaperone Hsp33
compare
AO353
_15250
-1.3
-3.5
cytochrome B
compare
AO353
_10455
-1.3
-2.3
tRNA modification GTPase MnmE
compare
AO353
_13605
-1.3
-6.5
oxidoreductase
compare
AO353
_21625
-1.3
-2.1
Fe/S biogenesis protein NfuA
compare
AO353
_05130
-1.2
-2.1
anti-anti-sigma factor
compare
AO353
_09890
-1.2
-4.5
phosphate ABC transporter ATP-binding protein
compare
AO353
_05145
-1.2
-5.3
ABC transporter permease
compare
AO353
_03015
-1.2
-4.7
arginine N-succinyltransferase
compare
AO353
_21655
-1.2
-4.0
cobalamin biosynthesis protein CobW
compare
AO353
_13610
-1.2
-2.4
precorrin isomerase
compare
AO353
_00330
-1.2
-6.9
hypothetical protein
compare
AO353
_20185
-1.2
-4.9
DNA-binding protein
compare
AO353
_15725
-1.2
-4.2
hypothetical protein
compare
AO353
_20630
-1.1
-5.7
SAM-dependent methyltransferase
compare
AO353
_00515
-1.1
-2.8
hypothetical protein
compare
AO353
_16450
-1.1
-7.3
protein-PII uridylyltransferase
compare
AO353
_10040
-1.1
-6.1
transcriptional regulator
compare
AO353
_00650
-1.1
-2.0
cytochrome biogenesis protein
compare
AO353
_05135
-1.1
-5.9
toluene tolerance protein
compare
AO353
_02635
-1.1
-6.3
cobyrinic acid a,c-diamide synthase
compare
AO353
_20370
-1.1
-3.2
MerR family transcriptional regulator
compare
AO353
_09260
-1.1
-4.8
ribonuclease PH
compare
AO353
_04175
-1.0
-3.2
glycerol uptake facilitator GlpF
compare
AO353
_19295
-1.0
-2.7
hypothetical protein
compare
AO353
_07315
-1.0
-2.1
rRNA methyltransferase
compare
AO353
_06155
-1.0
-5.8
copper-transporting ATPase
compare
AO353
_12345
-1.0
-1.9
twin-arginine protein translocation system subunit TatC
compare
AO353
_11165
-1.0
-5.2
hypothetical protein
compare
AO353
_00490
-1.0
-2.9
PasA protein
compare
AO353
_14795
-1.0
-3.6
molybdopterin-synthase adenylyltransferase
compare
AO353
_08505
-1.0
-6.7
FAD-linked oxidase
compare
AO353
_00430
-1.0
-3.0
glyceraldehyde-3-phosphate dehydrogenase
compare
AO353
_05140
-1.0
-5.2
outer membrane lipid asymmetry maintenance protein MlaD
compare
AO353
_22025
-1.0
-2.7
iron-sulfur cluster repair di-iron protein
compare
AO353
_03250
-1.0
-2.4
gluconokinase
compare
AO353
_10945
-1.0
-2.6
cytochrome C
compare
AO353
_11530
-1.0
-4.7
diguanylate cyclase
compare
AO353
_14405
-1.0
-6.2
ATPase
compare
AO353
_20410
-1.0
-2.1
hypothetical protein
compare
AO353
_15490
-1.0
-2.0
hypothetical protein
compare
AO353
_03425
-0.9
-5.4
glyceraldehyde-3-phosphate dehydrogenase
compare
AO353
_12240
-0.9
-3.7
histidine utilization repressor
compare
AO353
_13600
-0.9
-3.3
precorrin-6Y C5,15-methyltransferase
compare
AO353
_21640
-0.9
-1.3
cobalt transporter
compare
AO353
_20660
-0.9
-3.0
pseudouridine synthase
compare
AO353
_15905
-0.9
-4.9
outer membrane protein assembly factor BamB
compare
AO353
_04180
-0.9
-5.5
glycerol kinase
compare
AO353
_16825
-0.9
-3.8
hypothetical protein
compare
AO353
_08265
-0.9
-4.0
16S rRNA (guanine(966)-N(2))-methyltransferase RsmD
compare
AO353
_09045
-0.9
-2.2
hypothetical protein
compare
AO353
_22920
-0.9
-4.0
GntR family transcriptional regulator
compare
AO353
_00610
-0.9
-1.9
cytochrome C oxidase Cbb3
compare
AO353
_10510
-0.9
-1.3
benzoate transporter
compare
AO353
_03795
-0.9
-3.4
fumarate hydratase
compare
AO353
_18890
-0.9
-3.8
cytochrome C
compare
AO353
_05060
-0.9
-2.0
pressure-regulated protein
compare
AO353
_09900
-0.9
-5.9
phosphate ABC transporter permease
compare
AO353
_10430
-0.9
-3.0
chromosome partitioning protein ParB
compare
AO353
_03545
-0.9
-4.2
hypothetical protein
compare
AO353
_05325
-0.8
-3.2
GntR family transcriptional regulator
compare
AO353
_02615
-0.8
-4.4
cobyric acid synthase
compare
AO353
_04220
-0.8
-2.4
chemotaxis protein CheY
compare
AO353
_03395
-0.8
-4.9
sugar ABC transporter substrate-binding protein
compare
AO353
_09040
-0.8
-1.7
diaminopimelate epimerase
compare
AO353
_03245
-0.8
-4.3
permease DsdX
compare
AO353
_28380
-0.8
-3.0
GntR family transcriptional regulator
compare
AO353
_03390
-0.8
-5.7
sugar ABC transporter permease
compare
AO353
_08640
-0.8
-2.0
hypothetical protein
compare
AO353
_10985
-0.8
-1.5
hypothetical protein
compare
AO353
_02100
-0.8
-2.9
phosphoglycolate phosphatase
compare
AO353
_19145
-0.8
-2.6
protease HtpX
compare
AO353
_12935
-0.8
-2.5
glycosyltransferase
compare
AO353
_03025
-0.8
-3.3
acetylornithine aminotransferase
compare
AO353
_06460
-0.8
-4.6
hypothetical protein
compare
AO353
_06350
-0.8
-4.9
hydrogenase
compare
AO353
_09875
-0.8
-1.9
hypothetical protein
compare
Specific Phenotypes
None in this experiment
For Pseudomonas fluorescens FW300-N2E3 in pH experiments