Experiment set6IT058 for Pseudomonas fluorescens FW300-N2E3
Minimal media pH6
Group: pHMedia: RCH2_defined_glucose_MES, pH=6
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 10/28/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 20 mM MES sodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 6 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Terminal cytochrome C oxidases | 2 |
| Beta-Glucoside Metabolism | 1 |
| Biogenesis of cbb3-type cytochrome c oxidases | 1 |
| Potassium homeostasis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Oxidative phosphorylation
- Cyanoamino acid metabolism
- Starch and sucrose metabolism
- Phenylpropanoid biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| neolinustatin bioactivation | 3 | 2 | 2 |
| linustatin bioactivation | 4 | 2 | 2 |
| arsenite to oxygen electron transfer | 2 | 1 | 1 |
| linamarin degradation | 2 | 1 | 1 |
| lotaustralin degradation | 2 | 1 | 1 |
| arsenite to oxygen electron transfer (via azurin) | 3 | 1 | 1 |
| cellulose degradation II (fungi) | 3 | 1 | 1 |
| aerobic respiration I (cytochrome c) | 4 | 3 | 1 |
| aerobic respiration II (cytochrome c) (yeast) | 4 | 3 | 1 |
| coumarin biosynthesis (via 2-coumarate) | 5 | 2 | 1 |
| Fe(II) oxidation | 6 | 3 | 1 |
| α-tomatine degradation | 6 | 1 | 1 |
| firefly bioluminescence | 14 | 2 | 1 |