Experiment set6IT052 for Sphingomonas koreensis DSMZ 15582
L-Glutamic acid monopotassium salt monohydrate carbon source; no added vitamins
Group: nutrientMedia: RCH2_defined_noCarbon_noVitamins + L-Glutamic acid monopotassium salt monohydrate (10 mM), pH=7
Culturing: korea_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Adam on 15-Mar-20
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate)
Specific Phenotypes
For 9 genes in this experiment
For nutrient L-Glutamic acid monopotassium salt monohydrate in Sphingomonas koreensis DSMZ 15582
For nutrient L-Glutamic acid monopotassium salt monohydrate across organisms
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| incomplete reductive TCA cycle | 7 | 5 | 1 |
| TCA cycle V (2-oxoglutarate synthase) | 9 | 9 | 1 |
| TCA cycle VII (acetate-producers) | 9 | 7 | 1 |
| TCA cycle VI (Helicobacter) | 9 | 7 | 1 |
| reductive TCA cycle I | 11 | 8 | 1 |
| reductive TCA cycle II | 12 | 7 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 20 | 1 |