Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
Quality Metrics:
Time0 | 13-Feb-20 PseudoSBW25_INTG_ML3_set6 | which Time0s the sample was compared to |
cor12 | 0.24 | rank correlation(fit1, fit2), where fit1 is fitness for the first half (10-50%) and fit2 is fitness for the second half (50-90%) of each gene |
maxFit | 1.81 | The maximum fitness value |
opcor | 0.22 | rank correlation(upstream gene, downstream gene) over pairs that are adjacent and likely to be in the same operon |
adjcor | 0.01 | like opcor but for adjacent genes that are not on the same strand |
gccor | 0.09 | linear correlation of gene fitness and gene GC content |
mad12 | 0.16 | median absolute difference of fit1, fit2 |
mad12c | 1.04 | median absolute difference of log count for 1st and 2nd half of genes in this sample |
mad12c_t0 | 1.00 | like mad12c but for the Time0s |
gMed | 388 | median reads per gene in this sample |
gMedt0 | 1112 | median reads per gene in the Time0 sample |
gMean | 595 | mean reads per gene in this sample |
nMapped | 5.421 M | #reads for this sample that corresponded to a known strain (in millions) |
nPastEnd | 0.001 M | #reads that corresponded to a strain that has an insertion within the suicide vector instead of within the genome. |
nGenic | 3.266 M | #reads that lie within central 10-90% of a gene |
nUsed | 3.257 M | #reads used to estimate gene fitness (genic and enough coverage for strain and for gene) |
Specific Phenotypes
For 3 genes in this experiment
For soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs in Pseudomonas fluorescens SBW25-INTG
For soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs across organisms