Experiment set6IT045 for Azospirillum brasilense Sp245

Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB

Group: supernatant
Media: LB (0.4x) + supernatant from Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered (0.2 X)
Culturing: AzoBra_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Cat_Manuel_Trenton on 3/3/21
Media components: 4 g/L Tryptone, 2 g/L Yeast Extract, 2 g/L Sodium Chloride (final concentrations)

Specific Phenotypes

For 2 genes in this experiment

For supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered in Azospirillum brasilense Sp245

For supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered across organisms

SEED Subsystems

Subsystem	#Specific
LMPTP YfkJ cluster	1
Oxidative stress	1
Photorespiration (oxidative C2 cycle)	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Tryptophan metabolism
• Methane metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
superoxide radicals degradation	2	2	1
methanol oxidation to formaldehyde IV	2	1	1
ethanol degradation IV	3	3	1
reactive oxygen species degradation	4	4	1