Experiment set6IT045 for Klebsiella michiganensis M5al

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L-Threonine nitrogen source

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + L-Threonine (20 mM), pH=7
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Apr-17
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1809 B1

Specific Phenotypes

For 9 genes in this experiment

For nitrogen source L-Threonine in Klebsiella michiganensis M5al

For nitrogen source L-Threonine across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 3
Ethanolamine utilization 1
Glycine and Serine Utilization 1
Glycine cleavage system 1
Molybdenum cofactor biosynthesis 1
Photorespiration (oxidative C2 cycle) 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-alanine degradation III 1 1 1
L-alanine biosynthesis II 1 1 1
L-alanine degradation V (oxidative Stickland reaction) 2 2 1
L-alanine degradation II (to D-lactate) 3 3 1
molybdenum cofactor biosynthesis 3 3 1
glycine biosynthesis II 3 3 1
glycine cleavage 3 3 1
pyruvate fermentation to acetate and alanine 3 2 1
superpathway of L-alanine biosynthesis 4 4 1
bis(tungstenpterin) cofactor biosynthesis 4 1 1
ethanolamine utilization 5 5 1
molybdopterin biosynthesis 6 5 1
L-alanine degradation VI (reductive Stickland reaction) 6 4 1
anaerobic energy metabolism (invertebrates, cytosol) 7 5 1
superpathway of L-alanine fermentation (Stickland reaction) 9 7 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 8 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 9 1
superpathway of anaerobic energy metabolism (invertebrates) 17 12 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 23 1