Experiment set6IT037 for Dinoroseobacter shibae DFL-12

marine broth with Nalidixic acid sodium salt 0.048 mg/ml

Group: stress
Media: marine_broth_2216 + Nalidixic acid sodium salt (0.048 mg/ml)
Culturing: Dino_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 5/14/2014
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 922 F5,F6

Specific Phenotypes

For 2 genes in this experiment

For stress Nalidixic acid sodium salt in Dinoroseobacter shibae DFL-12

For stress Nalidixic acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, UvrABC system	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glutamate metabolism
• Arginine and proline metabolism
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamine biosynthesis I	1	1	1
ammonia assimilation cycle I	2	2	1
ammonia assimilation cycle II	2	2	1
ammonia assimilation cycle III	3	3	1
superpathway of ammonia assimilation (plants)	3	3	1
L-aspartate degradation III (anaerobic)	3	1	1
L-aspartate degradation II (aerobic)	3	1	1
L-glutamate and L-glutamine biosynthesis	7	6	1
L-arginine biosynthesis II (acetyl cycle)	10	10	1