Experiment set6IT033 for Bacteroides thetaiotaomicron VPI-5482
BHIS with Zinc Pyrithione 0.05 mM
Group: stressMedia: BHIS + Zinc Pyrithione (0.05 mM) + Dimethyl Sulfoxide (0.5 vol%)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Adam on 5/2/18
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin
Growth plate: 2025 D3
Specific Phenotypes
For 5 genes in this experiment
For stress Zinc Pyrithione in Bacteroides thetaiotaomicron VPI-5482
For stress Zinc Pyrithione across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Histidine metabolism
- beta-Alanine metabolism
- Urea cycle and metabolism of amino groups
- Alanine and aspartate metabolism
- Glycine, serine and threonine metabolism
- Cysteine metabolism
- Arginine and proline metabolism
- Glutathione metabolism
- Nicotinate and nicotinamide metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-serine degradation | 3 | 3 | 3 |
| L-tryptophan degradation II (via pyruvate) | 3 | 3 | 2 |
| D-serine degradation | 3 | 2 | 2 |
| L-cysteine degradation II | 3 | 2 | 2 |
| glutathione degradation (DUG pathway) | 2 | 1 | 1 |
| glycine betaine degradation III | 7 | 4 | 3 |
| felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis | 5 | 2 | 2 |
| glycine betaine degradation I | 8 | 4 | 3 |
| glycine degradation | 3 | 2 | 1 |
| L-methionine biosynthesis II | 6 | 3 | 2 |
| L-mimosine degradation | 8 | 4 | 2 |
| muropeptide degradation | 4 | 2 | 1 |
| glutathione-mediated detoxification I | 8 | 2 | 2 |
| NAD salvage pathway V (PNC V cycle) | 5 | 3 | 1 |
| γ-glutamyl cycle | 6 | 1 | 1 |
| NAD salvage pathway I (PNC VI cycle) | 7 | 5 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 12 | 2 |
| purine nucleobases degradation II (anaerobic) | 24 | 12 | 3 |
| NAD salvage (plants) | 11 | 5 | 1 |
| superpathway of NAD biosynthesis in eukaryotes | 14 | 6 | 1 |