Experiment set6IT026 for Bifidobacterium breve UCC2003

reconstituted MRS with Sialyllacto-N-tetraose a 5.0 mM

Group: carbon source
Media: reconstituted MRS + Sialyllacto-N-tetraose a (5 mM), pH=6.8
Culturing: Bifido_ML2, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Anthony Shiver on 7/15/2020

Specific Phenotypes

For 2 genes in this experiment

For carbon source Sialyllacto-N-tetraose a in Bifidobacterium breve UCC2003

For carbon source Sialyllacto-N-tetraose a across organisms

SEED Subsystems

Subsystem	#Specific
Chitin and N-acetylglucosamine utilization	1
Galactosylceramide and Sulfatide metabolism	1
Lactose and Galactose Uptake and Utilization	1
Lactose utilization	1
N-Acetyl-Galactosamine and Galactosamine Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Other glycan degradation
• Glycosaminoglycan degradation
• Glycosphingolipid biosynthesis - ganglio series
• Galactose metabolism
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - globo series

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
lactose degradation III	1	1	1
xyloglucan degradation II (exoglucanase)	8	3	3
chitin degradation II (Vibrio)	6	3	2
chitin degradation III (Serratia)	7	3	1
peptidoglycan recycling II	10	1	1
peptidoglycan recycling I	14	7	1