Experiment set6IT019 for Dinoroseobacter shibae DFL-12
marine broth with 1-ethyl-3-methylimidazolium acetate 2 mM
Group: stressMedia: marine_broth_2216 + 1-ethyl-3-methylimidazolium acetate (2 mM)
Culturing: Dino_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 1/15/2015
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: redo2 E5,E6
Specific Phenotypes
For 2 genes in this experiment
For stress 1-ethyl-3-methylimidazolium acetate in Dinoroseobacter shibae DFL-12
For stress 1-ethyl-3-methylimidazolium acetate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Purine Utilization | 1 |
| Purine conversions | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| guanosine nucleotides degradation III | 4 | 4 | 1 |
| guanosine nucleotides degradation II | 4 | 4 | 1 |
| superpathway of guanosine nucleotides degradation (plants) | 6 | 5 | 1 |
| drosopterin and aurodrosopterin biosynthesis | 7 | 4 | 1 |
| purine nucleotides degradation II (aerobic) | 11 | 11 | 1 |
| purine nucleotides degradation I (plants) | 12 | 10 | 1 |
| purine nucleobases degradation I (anaerobic) | 15 | 6 | 1 |
| superpathway of purines degradation in plants | 18 | 13 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 16 | 1 |