Experiment set6IT015 for Marinobacter adhaerens HP15
casamino acids
Group: carbon sourceMedia: DinoMM_noCarbon_HighNutrient + casamino acids (2 mg/ml), pH=7
Culturing: Marino_ML2, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 4.6 generations
By: Adam on 3/4/2014
Media components: 20 g/L Sea salts, 0.3 g/L Ammonium Sulfate, 0.1 g/L Potassium phosphate monobasic, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 2 genes in this experiment
For carbon source casamino acids in Marinobacter adhaerens HP15
For carbon source casamino acids across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Leucine Degradation and HMG-CoA Metabolism | 2 |
| Branched-Chain Amino Acid Biosynthesis | 1 |
| Isoleucine degradation | 1 |
| Valine degradation | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-threonine degradation V | 2 | 2 | 1 |
| 2-oxoisovalerate decarboxylation to isobutanoyl-CoA | 3 | 3 | 1 |
| 2-oxobutanoate degradation I | 4 | 2 | 1 |
| L-leucine degradation I | 6 | 6 | 1 |
| L-isoleucine degradation I | 6 | 5 | 1 |
| L-valine degradation I | 8 | 7 | 1 |
| superpathway of L-methionine salvage and degradation | 16 | 8 | 1 |
| even iso-branched-chain fatty acid biosynthesis | 34 | 25 | 1 |
| anteiso-branched-chain fatty acid biosynthesis | 34 | 25 | 1 |
| odd iso-branched-chain fatty acid biosynthesis | 34 | 25 | 1 |