Experiment set6IT014 for Klebsiella michiganensis M5al

L-Valine nitrogen source

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + L-Valine (20 mM), pH=7
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Apr-17
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1808 C2

Specific Phenotypes

For 3 genes in this experiment

For nitrogen source L-Valine in Klebsiella michiganensis M5al

For nitrogen source L-Valine across organisms

SEED Subsystems

Subsystem	#Specific
Benzoate degradation	1
Pyruvate Alanine Serine Interconversions	1
Respiratory dehydrogenases 1	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Phenylalanine metabolism
• Benzoate degradation via hydroxylation
• Toluene and xylene degradation
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-alanine degradation III	1	1	1
L-alanine biosynthesis II	1	1	1
L-alanine degradation V (oxidative Stickland reaction)	2	2	1
L-alanine degradation I	2	2	1
L-alanine degradation II (to D-lactate)	3	3	1
pyruvate fermentation to acetate and alanine	3	2	1
D-phenylglycine degradation	3	1	1
superpathway of L-alanine biosynthesis	4	4	1
mandelate degradation I	5	2	1
L-alanine degradation VI (reductive Stickland reaction)	6	4	1
4-hydroxymandelate degradation	6	3	1
anaerobic energy metabolism (invertebrates, cytosol)	7	5	1
indole-3-acetate degradation II	7	1	1
superpathway of L-alanine fermentation (Stickland reaction)	9	7	1
C4 photosynthetic carbon assimilation cycle, NAD-ME type	11	8	1
C4 photosynthetic carbon assimilation cycle, PEPCK type	14	9	1
superpathway of anaerobic energy metabolism (invertebrates)	17	12	1
superpathway of aromatic compound degradation via 3-oxoadipate	35	23	2
mandelate degradation to acetyl-CoA	18	14	1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate	42	17	2
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	23	1