Experiment set6IT009 for Desulfovibrio vulgaris Hildenborough JW710

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MoYLS4 with FCCP 10uM

Group: stress
Media: MoYLS4 + FCCP (10 uM), pH=7.2
Culturing: DvH_JW710, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Valentine on 12/12/2016
Media components: 1 g/L Yeast Extract, 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, 15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate, Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 23 genes in this experiment

For stress FCCP in Desulfovibrio vulgaris Hildenborough JW710

For stress FCCP across organisms

SEED Subsystems

Subsystem #Specific
Molybdenum cofactor biosynthesis 6
Glycerol and Glycerol-3-phosphate Uptake and Utilization 2
Multidrug Resistance Efflux Pumps 2
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) 2
Arginine and Ornithine Degradation 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Glycine reductase, sarcosine reductase and betaine reductase 1
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria 1
Nitrosative stress 1
Respiratory dehydrogenases 1 1
Rubrerythrin 1
Thioredoxin-disulfide reductase 1
Transport of Molybdenum 1
Wyeosine-MimG Biosynthesis 1
YcfH 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
glycerophosphodiester degradation 2 2 2
glycerol-3-phosphate shuttle 2 2 1
glycerol-3-phosphate to fumarate electron transfer 2 2 1
glycerol and glycerophosphodiester degradation 4 3 2
glycerol-3-phosphate to hydrogen peroxide electron transport 2 1 1
nitrate reduction IX (dissimilatory) 2 1 1
thioredoxin pathway 2 1 1
glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer 2 1 1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer 2 1 1
phosphatidylcholine resynthesis via glycerophosphocholine 2 1 1
glycerol degradation I 3 2 1
sn-glycerol 3-phosphate anaerobic respiration 3 1 1
phospholipid remodeling (phosphatidylethanolamine, yeast) 4 1 1
pentose phosphate pathway (non-oxidative branch) II 6 6 1
glycolysis IV 10 8 1
anandamide biosynthesis I 12 3 1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle 22 14 1