Experiment set6IT006 for Desulfovibrio vulgaris Hildenborough JW710

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MoYLS4 with FCCP 2uM

Group: stress
Media: MoYLS4 + FCCP (2 uM), pH=7.2
Culturing: DvH_JW710, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Valentine on 12/12/2016
Media components: 1 g/L Yeast Extract, 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, 15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate, Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 25 genes in this experiment

For stress FCCP in Desulfovibrio vulgaris Hildenborough JW710

For stress FCCP across organisms

SEED Subsystems

Subsystem #Specific
Molybdenum cofactor biosynthesis 5
Glycerol and Glycerol-3-phosphate Uptake and Utilization 2
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 2
Multidrug Resistance Efflux Pumps 2
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) 2
Nitrosative stress 2
Bacterial Cell Division 1
MLST 1
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria 1
Potassium homeostasis 1
Protein chaperones 1
Respiratory dehydrogenases 1 1
Rubrerythrin 1
Transport of Molybdenum 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
glycerol degradation I 3 2 2
glycerol-3-phosphate shuttle 2 2 1
glycerol-3-phosphate to fumarate electron transfer 2 2 1
glycerophosphodiester degradation 2 2 1
glycerol and glycerophosphodiester degradation 4 3 2
nitrate reduction IX (dissimilatory) 2 1 1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer 2 1 1
glycerol-3-phosphate to hydrogen peroxide electron transport 2 1 1
glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer 2 1 1
sn-glycerol 3-phosphate anaerobic respiration 3 1 1