Experiment set6IT005 for Dinoroseobacter shibae DFL-12

marine broth with Cephalothin sodium salt 0.0001 mg/ml

Group: stress
Media: marine_broth_2216 + Cephalothin sodium salt (1e-04 mg/ml)
Culturing: Dino_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 5/14/2014
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 919 D5,D6

Specific Phenotypes

For 12 genes in this experiment

For stress Cephalothin sodium salt in Dinoroseobacter shibae DFL-12

For stress Cephalothin sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Xylose utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
peptidoglycan recycling II	10	2	1
peptidoglycan recycling I	14	7	1