Experiment set6IT004 for Phaeobacter inhibens DSM 17395
marine broth with Paraquat dichloride 0.016 mg/ml
Group: stressMedia: marine_broth_2216 + Paraquat dichloride (0.016 mg/ml)
Culturing: Phaeo_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on marchapr14
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 830 B3,B4
Specific Phenotypes
For 6 genes in this experiment
For stress Paraquat dichloride in Phaeobacter inhibens DSM 17395
For stress Paraquat dichloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Choline and Betaine Uptake and Betaine Biosynthesis | 3 |
| Choline Transport | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| choline-O-sulfate degradation | 3 | 3 | 3 |
| choline degradation I | 2 | 2 | 2 |
| glycine betaine biosynthesis I (Gram-negative bacteria) | 2 | 2 | 2 |
| glycine betaine biosynthesis II (Gram-positive bacteria) | 2 | 2 | 2 |
| β-alanine biosynthesis IV | 2 | 1 | 1 |
| β-alanine biosynthesis I | 2 | 1 | 1 |
| glycine betaine biosynthesis III (plants) | 3 | 2 | 1 |
| dimethylsulfoniopropanoate biosynthesis I (Wollastonia) | 3 | 1 | 1 |
| choline degradation IV | 4 | 2 | 1 |
| dimethylsulfoniopropanoate biosynthesis II (Spartina) | 4 | 1 | 1 |