Experiment set6IT003 for Desulfovibrio vulgaris Hildenborough JW710

Compare to:

Pyruvate-Thiosulfate (60-15 mM) + 0.1% Yeast Extract

Group: respiratory growth
Media: Dv_base_Y_medium + Sodium pyruvate (60 mM) + Sodium thiosulfate (15 mM), pH=7.2
Culturing: DvH_JW710, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Valentine on 12/12/2016
Media components: 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, 1 g/L Yeast Extract, Desulfovibrio trace elements (15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate), Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 1 genes in this experiment

For respiratory growth Sodium pyruvate in Desulfovibrio vulgaris Hildenborough JW710

For respiratory growth Sodium pyruvate across organisms

SEED Subsystems

Subsystem #Specific
De Novo Purine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate 2 2 1
5-aminoimidazole ribonucleotide biosynthesis I 5 5 1
superpathway of 5-aminoimidazole ribonucleotide biosynthesis 6 6 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 8 1
superpathway of purine nucleotides de novo biosynthesis I 21 21 1