Experiment set6IT003 for Bacteroides thetaiotaomicron VPI-5482

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BHIS with Thioridazine hydrochloride 0.0125 mM

Group: stress
Media: BHIS + Thioridazine hydrochloride (0.0125 mM) + Dimethyl Sulfoxide (0.125 vol%)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Adam on 5/2/18
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin
Growth plate: 2023 B4

Specific Phenotypes

For 9 genes in this experiment

For stress Thioridazine hydrochloride in Bacteroides thetaiotaomicron VPI-5482

For stress Thioridazine hydrochloride across organisms

SEED Subsystems

Subsystem #Specific
ATP-dependent RNA helicases, bacterial 1
Acetyl-CoA fermentation to Butyrate 1
De Novo Purine Biosynthesis 1
Isoleucine degradation 1
Protein chaperones 1
Valine degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
pyruvate fermentation to butanoate 7 3 2
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 5 2
5-aminoimidazole ribonucleotide biosynthesis I 5 5 1
5-aminoimidazole ribonucleotide biosynthesis II 5 5 1
(S)-propane-1,2-diol degradation 5 3 1
superpathway of 5-aminoimidazole ribonucleotide biosynthesis 6 6 1
L-threonine degradation I 6 5 1
L-glutamate degradation VII (to butanoate) 12 4 2
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 6 2
superpathway of L-threonine metabolism 18 13 1
superpathway of purine nucleotides de novo biosynthesis I 21 21 1
superpathway of purine nucleotides de novo biosynthesis II 26 23 1
superpathway of histidine, purine, and pyrimidine biosynthesis 46 42 1