Experiment set6H41 for Pseudomonas stutzeri RCH2

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RCH2 defined media with Sodium Chlorate 40 mM

Group: stress
Media: RCH2_defined_no_vitamin + Sodium Chlorate (40 mM), pH=7.2
Culturing: psRCH2_ML7, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Kelly on 8/12/2013
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM Sodium D,L-Lactate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate)
Growth plate: 627 C7,C8

Specific Phenotypes

For 12 genes in this experiment

For stress Sodium Chlorate in Pseudomonas stutzeri RCH2

For stress Sodium Chlorate across organisms

SEED Subsystems

Subsystem #Specific
Chorismate Synthesis 2
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) 2
Hemin transport system 1
Molybdenum cofactor biosynthesis 1
N-linked Glycosylation in Bacteria 1
Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
3-dehydroquinate biosynthesis I 2 2 1
guanosine ribonucleotides de novo biosynthesis 4 4 1
UDP-N-acetyl-β-L-fucosamine biosynthesis 4 1 1
UDP-N-acetyl-β-L-quinovosamine biosynthesis 4 1 1
methylphosphonate degradation I 5 4 1
dZTP biosynthesis 5 3 1
CMP-pseudaminate biosynthesis 6 6 1
superpathway of guanosine nucleotides de novo biosynthesis I 6 6 1
chorismate biosynthesis I 7 7 1
glyphosate degradation III 7 4 1
CMP-diacetamido-8-epilegionaminic acid biosynthesis 7 1 1
superpathway of guanosine nucleotides de novo biosynthesis II 8 7 1
(aminomethyl)phosphonate degradation 8 6 1
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 10 1
superpathway of L-tryptophan biosynthesis 13 13 1
superpathway of purine nucleotide salvage 14 11 1
superpathway of aromatic amino acid biosynthesis 18 18 1
superpathway of purine nucleotides de novo biosynthesis I 21 21 1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis 24 9 1
superpathway of purine nucleotides de novo biosynthesis II 26 24 1
superpathway of histidine, purine, and pyrimidine biosynthesis 46 44 1
superpathway of chorismate metabolism 59 44 1